Anti-Hemojuvelin antibody [C5] (ab239497)
Key features and details
- Mouse monoclonal [C5] to Hemojuvelin
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Hemojuvelin antibody [C5]
See all Hemojuvelin primary antibodies -
Description
Mouse monoclonal [C5] to Hemojuvelin -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment (His-tag) corresponding to Human Hemojuvelin aa 200 to the C-terminus. N-terminal His-tag (Expressed in E.coli).
Database link: Q6ZVN8 -
Positive control
- IHC-P: Human kidney, stomach, glioma and liver tissues. WB: Rat liver lysate; Recombinant human Hemojuvelin protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C5 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab239497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 45 kDa.
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IHC-P |
Use a concentration of 5 - 30 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 45 kDa. |
IHC-P
Use a concentration of 5 - 30 µg/ml. |
Target
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Relevance
Mutations in Repulsive Guidance Molecule (also known as RGM-C; HJV; hemojuvelin; Hfe2) cause juvenile hemochromatosis, a severe iron overload disease. RGM-C gene expression has been characterized in the developing mouse and found to be exclusively expressed in all striated muscle and in the myocardium. -
Cellular localization
Cell membrane; Lipid-anchor, GPI-anchor -
Database links
- Entrez Gene: 148738 Human
- Entrez Gene: 310681 Rat
- Omim: 608374 Human
- SwissProt: Q6ZVN8 Human
- SwissProt: Q8N7M5 Rat
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Alternative names
- 2310035L15Rik antibody
- 5230400G09Rik antibody
- AI414844 antibody
see all
Images
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Paraffin-embedded human kidney tissue stained for Hemojuvelin using ab239497 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
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Anti-Hemojuvelin antibody [C5] (ab239497) at 2 µg/ml + Rat liver lysate
Secondary
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 45 kDa -
Paraffin-embedded human stomach tissue stained for Hemojuvelin using ab239497 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
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Paraffin-embedded human glioma tissue stained for Hemojuvelin using ab239497 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
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Paraffin-embedded human liver tissue stained for Hemojuvelin using ab239497 at 30 µg/ml in immunohistochemical analysis. DAB staining. HRP-Goat Anti-Mouse IgG secondary antibody used at 2 µg/ml.
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Anti-Hemojuvelin antibody [C5] (ab239497) at 2 µg/ml + Recombinant human Hemojuvelin protein
Secondary
HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 45 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab239497 has not yet been referenced specifically in any publications.