Key features and details
- Mouse monoclonal [C6] to Hemojuvelin
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG2b
Product nameAnti-Hemojuvelin antibody [C6]
See all Hemojuvelin primary antibodies
DescriptionMouse monoclonal [C6] to Hemojuvelin
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Rat, Human, Pig
Recombinant fragment (His-tag) corresponding to Human Hemojuvelin aa 234-416. Expressed in E.coli.
KVYQAEVDNLPVAFEDGSINGGDRPGGSSLSIQTANPGNHVEIQAAYIGT TIIIRQTAGQLSFSIKVAEDVAMAFSAEQDLQLCVGGCPPSQRLSRSERN RRGAITIDTARRLCKEGLPVEDAYFHSCVFDVLISGDPNFTVAAQAALED ARAFLPDLEKLHLFPSDAGVPLSSATLLAPLLS
Database link: Q6ZVN8
- WB: Recombinant human Hemojuvelin; Pig liver lysate; Rat liver lysate. IHC-P: Human liver, kidney, glioma and prostate gland cancer tissue.
This product was previously labelled as Repulsive Guidance Molecule C
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS.
Light chain typekappa
Our Abpromise guarantee covers the use of ab239495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 45 kDa.|
|IHC-P||Use a concentration of 5 - 30 µg/ml.|
RelevanceMutations in Repulsive Guidance Molecule (also known as RGM-C; HJV; hemojuvelin; Hfe2) cause juvenile hemochromatosis, a severe iron overload disease. RGM-C gene expression has been characterized in the developing mouse and found to be exclusively expressed in all striated muscle and in the myocardium.
Cellular localizationCell membrane; Lipid-anchor, GPI-anchor
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Anti-Hemojuvelin antibody [C6] (ab239495) at 2 µg/ml + Recombinant human Hemojuvelin protein
HRP-linked Rabbit anti-Mouse IgG at 1/5000 dilution
Predicted band size: 45 kDa
All lanes : Anti-Hemojuvelin antibody [C6] (ab239495) at 2 µg/ml
Lane 1 : Pig liver lysate
Lane 2 : Rat liver lysate
All lanes : HRP-Linked Rabbit Anti-Mouse IgG at 1/5000 dilution
Predicted band size: 45 kDa
Paraffin-embedded human liver tissue stained for Hemojuvelin using ab239495 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for Hemojuvelin using ab239495 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human glioma tissue stained for Hemojuvelin using ab239495 at 30 μg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human prostate cancer tissue stained for Hemojuvelin using ab239495 at 30 μg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab239495 has not yet been referenced specifically in any publications.