Key features and details
- Rabbit polyclonal to Hemopexin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-Hemopexin antibody
See all Hemopexin primary antibodies
DescriptionRabbit polyclonal to Hemopexin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Rabbit, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human Hemopexin aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Mouse Sciatic Nerve; Rat Sciatic Nerve; Mouse Liver; Rat Liver.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab133415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 52 kDa).|
FunctionBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Tissue specificityExpressed by the liver and secreted in plasma.
Sequence similaritiesBelongs to the hemopexin family.
Contains 5 hemopexin-like domains.
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
- Information by UniProt
- Beta 1B glycoprotein antibody
- Beta-1B-glycoprotein antibody
- FLJ56652 antibody
All lanes : Anti-Hemopexin antibody (ab133415) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Tissue Lysate
Lane 2 : Rat Sciatic Nerve Rat Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 12 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Hemopexin has been shown to migrate at ~70 kDa under reducing conditions by Madore et al PMID: 9930744. We welcome customer feedback. Hemopexin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133415 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab133415 has not yet been referenced specifically in any publications.