Overview

  • Product name

  • Description

    Rabbit polyclonal to Hemopexin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Rabbit, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Hemopexin aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab151532)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse Sciatic Nerve; Rat Sciatic Nerve; Mouse Liver; Rat Liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab133415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 52 kDa).

Target

Images

  • All lanes : Anti-Hemopexin antibody (ab133415) at 1 µg/ml

    Lane 1 : Mouse Sciatic Nerve Tissue Lysate
    Lane 2 : Rat Sciatic Nerve Rat Tissue Lysate
    Lane 3 : Liver (Mouse) Tissue Lysate
    Lane 4 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 52 kDa
    Observed band size: 72 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 12 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    Hemopexin has been shown to migrate at ~70 kDa under reducing conditions by Madore et al PMID: 9930744. We welcome customer feedback. Hemopexin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133415 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab133415 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab133415.
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