Anti-Hemopexin antibody (ab48135)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Hemopexin antibody
See all Hemopexin primary antibodies -
DescriptionRabbit polyclonal to Hemopexin
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Host speciesRabbit
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Tested applicationsSuitable for: RIA, WB, Sandwich ELISA, ELISAmore details
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Species reactivityReacts with: Human
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Immunogen
Full length native protein (purified) (Human)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol, PBS, pH 7.5 -
Concentration information loading... -
PurityProtein G purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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sELISA pair antibody
Applications
Our Abpromise guarantee covers the use of ab48135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| RIA | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. Predicted molecular weight: 51 kDa. | |
| Sandwich ELISA | Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [32] to Hemopexin (ab27711). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [32] to Hemopexin (ab27711) as Capture. | |
| ELISA | Use at an assay dependent dilution. |
Target
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FunctionBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
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Tissue specificityExpressed by the liver and secreted in plasma.
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Sequence similaritiesBelongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
Post-translational
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 3263 Human
- Omim: 142290 Human
- SwissProt: P02790 Human
- Unigene: 426485 Human
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Alternative names
- Beta 1B glycoprotein antibody
- Beta-1B-glycoprotein antibody
- FLJ56652 antibody
see all
Images
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Sandwich ELISA - Anti-Hemopexin antibody (ab48135)Standard Curve for Hemopexin (Analyte: Hemopexin protein (ab98988)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [32] to Hemopexin (ab27711) at 5µg/ml and Detector Antibody Rabbit polyclonal to Hemopexin (ab48135) at 0.5µg/ml.
Protocols
References
ab48135 has not yet been referenced specifically in any publications.
