Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Tissue lysate - whole (hu liver, hu colon, hu colon carcinoma & bovine li)
Specification
hu liver, hu colon, hu colon carcinoma & bovine li
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Diluent: on shaker in 5% milk
Dilution
1/10000
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Bands
Specific: ~75 & degradation ba kDa
Exposure
20 minute(s) and 0 second(s)
Positive control
human liver
Additional data
Additional Notes
Our results yield that this antibody is negative with bovine tissue and thus gives you a new negative species for the antibody data sheet.
Loading specifications of each lane.
Lane 1 = human liver;
Lane 2 = human colon;
Lane 3 = human colon carcinoma;
Lane 4 = bovine liver.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Steffen Rickelt
Verified customer
Submitted Jan 08 2014