Anti-HEPACAM antibody [EPR11217] (Monoclonal) (ab172635)

Product name

Anti-HEPACAM antibody [EPR11217]
See all HEPACAM primary antibodies
Description

Rabbit monoclonal [EPR11217] to HEPACAM
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human HEPACAM aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14CZ8
Positive control
- Human fetal kidney, fetal liver, fetal brain and stomach lysates; HepG2 cells.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11217
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab172635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 46 kDa).
ICC/IF		1/250 - 1/500.

Application notes

Is unsuitable for IHC-P or IP.

Function

Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.
Sequence similarities

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Domain

The cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility.
Post-translational
modifications

N-glycosylated.
Cellular localization

Cytoplasm. Membrane. In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.
Target information above from: UniProt accession Q14CZ8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 220296 Human
- Omim: 611642 Human
- SwissProt: Q14CZ8 Human
- Unigene: 159863 Human
Alternative names
- FLJ25530 antibody
- GlialCAM antibody
- HECAM_HUMAN antibody
- HEPACAM antibody
- Hepatocyte cell adhesion molecule antibody
- Protein hepaCAM antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-HEPACAM antibody [EPR11217] (ab172635)

Immunofluorescent analysis of HepG2 cells labeling HEPACAM with ab172635 at 1/250 dilution. DAPI nuclear staining (blue).
Western blot - Anti-HEPACAM antibody [EPR11217] (ab172635)

All lanes : Anti-HEPACAM antibody [EPR11217] (ab172635) at 1/1000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human stomach lysate
Lane 4 : Human fetal brain lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 46 kDa

ab172635 has not yet been referenced specifically in any publications.

Publishing research using ab172635? Please let us know so that we can cite the reference in this datasheet.

Anti-HEPACAM antibody [EPR11217] (ab172635)