Anti-HEPACAM antibody [EPR11217] (ab172635)
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Overview
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Product nameAnti-HEPACAM antibody [EPR11217]
See all HEPACAM primary antibodies -
DescriptionRabbit monoclonal [EPR11217] to HEPACAM
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human HEPACAM aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14CZ8 -
Positive control
- Human fetal kidney, fetal liver, fetal brain and stomach lysates; HepG2 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR11217
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab172635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 46 kDa). | |
| ICC/IF | 1/250 - 1/500. |
Target
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FunctionInvolved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.
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Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
DomainThe cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility.
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Post-translational
modificationsN-glycosylated. -
Cellular localizationCytoplasm. Membrane. In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.
- Information by UniProt
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Database links
- Entrez Gene: 220296 Human
- Omim: 611642 Human
- SwissProt: Q14CZ8 Human
- Unigene: 159863 Human
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Alternative names
- FLJ25530 antibody
- GlialCAM antibody
- HECAM_HUMAN antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-HEPACAM antibody [EPR11217] (ab172635)](https://www.abcam.com/ps/products/172/ab172635/Images/ab172635-195121-ab1726352.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HEPACAM antibody [EPR11217] (ab172635)Immunofluorescent analysis of HepG2 cells labeling HEPACAM with ab172635 at 1/250 dilution. DAPI nuclear staining (blue).
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![Western blot - Anti-HEPACAM antibody [EPR11217] (ab172635)](https://www.abcam.com/ps/products/172/ab172635/Images/ab172635-195120-ab1726351.jpg)
Western blot - Anti-HEPACAM antibody [EPR11217] (ab172635)All lanes : Anti-HEPACAM antibody [EPR11217] (ab172635) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human stomach lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa
References
ab172635 has not yet been referenced specifically in any publications.
