Overview

  • Product name
    Anti-HEPACAM antibody [EPR11217]
    See all HEPACAM primary antibodies
  • Description
    Rabbit monoclonal [EPR11217] to HEPACAM
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HEPACAM aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q14CZ8

  • Positive control
    • Human fetal kidney, fetal liver, fetal brain and stomach lysates; HepG2 cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 46 kDa).
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.
    • Sequence similarities
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Domain
      The cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localization
      Cytoplasm. Membrane. In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ25530 antibody
      • GlialCAM antibody
      • HECAM_HUMAN antibody
      • HEPACAM antibody
      • Hepatocyte cell adhesion molecule antibody
      • Protein hepaCAM antibody
      see all

    Images

    • Immunofluorescent analysis of HepG2 cells labeling HEPACAM with ab172635 at 1/250 dilution. DAPI nuclear staining (blue).

    • All lanes : Anti-HEPACAM antibody [EPR11217] (ab172635) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : Human stomach lysate
      Lane 4 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 46 kDa

    References

    ab172635 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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