RecombinantRabMAb

Recombinant Anti-HEPACAM antibody [EPR11217] (ab172635)

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Immunocytochemistry/ Immunofluorescence - Anti-HEPACAM antibody [EPR11217] (ab172635)
  • Western blot - Anti-HEPACAM antibody [EPR11217] (ab172635)
  • Anti-HEPACAM antibody [EPR11217] (ab172635)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11217] to HEPACAM
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-HEPACAM antibody [EPR11217]
    See all HEPACAM primary antibodies

  • Description

    Rabbit monoclonal [EPR11217] to HEPACAM

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HEPACAM aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q14CZ8

  • Positive control

    • Human fetal kidney, fetal liver, fetal brain and stomach lysates; HepG2 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 46 kDa).
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 46 kDa).
ICC/IF
1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation.

    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • Domain

      The cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility.

    • Post-translational
      modifications

      N-glycosylated.

    • Cellular localization

      Cytoplasm. Membrane. In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1.
    • Information by UniProt

    • Database links

      • Alternative names

        • FLJ25530 antibody
        • GlialCAM antibody
        • HECAM_HUMAN antibody
        • HEPACAM antibody
        • Hepatocyte cell adhesion molecule antibody
        • Protein hepaCAM antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-HEPACAM antibody [EPR11217] (ab172635)
        Immunocytochemistry/ Immunofluorescence - Anti-HEPACAM antibody [EPR11217] (ab172635)

        Immunofluorescent analysis of HepG2 cells labeling HEPACAM with ab172635 at 1/250 dilution. DAPI nuclear staining (blue).

      • Western blot - Anti-HEPACAM antibody [EPR11217] (ab172635)
        Western blot - Anti-HEPACAM antibody [EPR11217] (ab172635)
        All lanes : Anti-HEPACAM antibody [EPR11217] (ab172635) at 1/1000 dilution

        Lane 1 : Human fetal kidney lysate
        Lane 2 : Human fetal liver lysate
        Lane 3 : Human stomach lysate
        Lane 4 : Human fetal brain lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 46 kDa

      • Anti-HEPACAM antibody [EPR11217] (ab172635)
        Anti-HEPACAM antibody [EPR11217] (ab172635)

      References (0)

      Publishing research using ab172635? Please let us know so that we can cite the reference in this datasheet.

      ab172635 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      Immunocytochemistry abreview for Anti-HEPACAM antibody [EPR11217]

       Excellent
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Human Cultured Cells (iPSC-derived astrocytes)
      Permeabilization
      No
      Specification
      iPSC-derived astrocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 14 2020

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