Recombinant Anti-HEPACAM antibody [EPR11217] (ab172635)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11217] to HEPACAM
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-HEPACAM antibody [EPR11217]
See all HEPACAM primary antibodies -
Description
Rabbit monoclonal [EPR11217] to HEPACAM -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HEPACAM aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q14CZ8 -
Positive control
- Human fetal kidney, fetal liver, fetal brain and stomach lysates; HepG2 cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11217 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 46 kDa).
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 46 kDa). |
ICC/IF
1/250 - 1/500. |
Target
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Function
Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
The cytoplasmic domain plays an important role in regulation of cell-matrix adhesion and cell motility. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cytoplasm. Membrane. In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1. - Information by UniProt
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Database links
- Entrez Gene: 220296 Human
- Omim: 611642 Human
- SwissProt: Q14CZ8 Human
- Unigene: 159863 Human
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Alternative names
- FLJ25530 antibody
- GlialCAM antibody
- HECAM_HUMAN antibody
see all
Images
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Immunofluorescent analysis of HepG2 cells labeling HEPACAM with ab172635 at 1/250 dilution. DAPI nuclear staining (blue).
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All lanes : Anti-HEPACAM antibody [EPR11217] (ab172635) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human stomach lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 46 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab172635 has not yet been referenced specifically in any publications.