Product nameAnti-HEPACAM2 antibody
See all HEPACAM2 primary antibodies
DescriptionRabbit polyclonal to HEPACAM2
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog
- HepG2 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab82977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1/62500.
Tissue specificityWidely expressed.
Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationMembrane. Golgi apparatus. Cytoplasm > cytoskeleton > spindle. In interphase, localized in the Golgi apparatus. During mitosis, associated with mitotic splindles and centrosomes.
- Information by UniProt
- AI987662 antibody
- HECA2_HUMAN antibody
- HEPACAM 2 antibody
Anti-HEPACAM2 antibody (ab82977) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
ab82977 has not yet been referenced specifically in any publications.