Overview

  • Product name
    Anti-Heparan Sulfate Proteoglycan 2 antibody [A74]
    See all Heparan Sulfate Proteoglycan 2 primary antibodies
  • Description
    Mouse monoclonal [A74] to Heparan Sulfate Proteoglycan 2
  • Host species
    Mouse
  • Specificity
    ab23418 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, IP, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Corneal endothelial cells (Cow).

  • Epitope
    Epitope is located in domain V of Perlecan.

Properties

Applications

Our Abpromise guarantee covers the use of ab23418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22278369
ICC/IF Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 469 kDa.
IP Use at an assay dependent concentration.
IHC-Fr 1/100.
ELISA 1/2000.
AP Use at an assay dependent concentration. Can be used to separate recombinant domain I from full length perlecan.

Target

  • Function
    Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
    Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
    The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.
  • Tissue specificity
    Found in the basement membranes.
  • Involvement in disease
    Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
    Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.
  • Sequence similarities
    Contains 4 EGF-like domains.
    Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 11 laminin EGF-like domains.
    Contains 3 laminin G-like domains.
    Contains 3 laminin IV type A domains.
    Contains 4 LDL-receptor class A domains.
    Contains 1 SEA domain.
  • Post-translational
    modifications
    Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
    N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basement membrane specific heparan sulfate proteoglycan core protein antibody
    • Endorepellin (domain V region) antibody
    • Heparan sulfate proteoglycan of basement membrane antibody
    • HSPG 2 antibody
    • HSPG antibody
    • Hspg2 antibody
    • LG3 peptide antibody
    • Perlecan antibody
    • PGBM_HUMAN antibody
    • PLC antibody
    • Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) antibody
    • SJA antibody
    • SJS antibody
    • SJS1 antibody
    see all

Images

  • Immunohistochemical analysis of Human ovary tissue, staining Heparan Sulfate Proteoglycan 2 with ab23418.

    Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/10 in diluent) for 30 minutes. An HRP-conjugated anti-mouse IgG was used as the secondary antibody.

    See Abreview

  • ab23418 staining Heparan Sulfate Proteoglycan 2 in Human fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with methanol, permeabilized with 1% Triton X-100 and blocked with 5% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/600 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse monoclonal (1/200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • McGarrity S  et al. Metabolic systems analysis of LPS induced endothelial dysfunction applied to sepsis patient stratification. Sci Rep 8:6811 (2018). WB ; Human . Read more (PubMed: 29717213) »
  • Blache U  et al. Notch-inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate. EMBO Rep 19:N/A (2018). Read more (PubMed: 29967223) »
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Question

Dear Sir/Madam, We are working on a laboratory located on Viet Nam and looking at Human Heparan Sulfate Measurements in dengue patient plasma. In the past, we ordered the Heparan Sulfate ELISA Kits from Seikagaku Company (Japan) to do researches related to Heparan Sulfate, these kits worked well for our experiments. However, these kits are no longer produced anymore. So, we are planning to design an ELISA protocol by ourselves for the researches in future. We found that your company has some monoclonal antibodies could be used for our experiments. We would like to ask you some information about these products. Could you please help us for the information? The products named anti –Heparan Sulfate Proteoglycan 2 antibody –A76 (ab26265) and anti-Heparan Sulfate Proteoglycan 2 antibody- A74 (ab23418) that could be used to detect for all kind of Heparan sulfate or only specific to HS Proteoglycan 2? What kind of the monoclonal antibody (ab 266265 or ab 23418) is more specific to HS if we plan to use these antibodies for coating the plates? What is the Epitope of ab 26265 antibody? It doesn’t be shown in the product instruction. The product called anti-Heparan sulfate Proteoglycan 2 antibody –A7L6 (Biotin) (ab79465) could be used for the ELISA test? This application doesn’t be suggested in the product instruction. The antibody ab79465 (Biotin) could be used as a biotinylated antibody for the ELISA plates pre- coated ab26265 or ab23418 , then it will be detected by HRP-Conjugated Streptavidin? I am looking forward to your reply as soon as possible. Please have a look. Thank you very much.  

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Answer

Thank you once again for your enquiries. I was in touch with our source for these antibodies again today, and I am pleased I now have the information for you.   1. Can ab26265 and ab23418 be used to detect for all kind of Heparan sulfate or are they only specific to HS Proteoglycan 2? As far as we are aware. these antibodies are specific to HS Proteoglycan 2, but this has not been tested in the laboratory. If the epitope is in one of the domains that exists in the other heparan sulfates it might detect these,  however I am sorry the epitope has not been mapped. 2. Which is more specific to HS if we plan to use these antibodies for coating plates in ELISA? The product specification states:ab26265 is more sensitive than ab23418 in ELISA. 3. What is the Epitope of ab26265 antibody? I am sorry the epitope has not been mapped. 4. ab79465 could be used for the ELISA test? I am sorry to confirm this antibody has not been tested in ELISA.  All tested and guaranteed species and applications are listed on the online datasheet and updated as soon as we receive new information. If you would like to test this antibody in ELISA, please letme know before you purchase as you may be eligable for a testing discount. 5. I am sorry to confirm these antibodies  have not been tested together in sandwich ELISA (sELISA) and are not guaranteed for this application.  ab26265 and ab23418 have been tested in indirect or direct ELISA only. I hope this information will be helpful to you. Thank you for your patience, and if you have any further questions, or if you would like to test any of these antibodies in an untestd appcliation,  please do not hesitate to contact me.  

Read More
Application
Flow Cytometry
Sample
Rhesus monkey Cell (chorioretinal endothelial cell)
Permeabilization
No
Gating Strategy
size and granularity
Specification
chorioretinal endothelial cell
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with Accutase
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde

Andrea Cabrera

Verified customer

Submitted Sep 22 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human decelluarized adipose matrix)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate buffer pH 6.0 for 40 minutes at 97.0 ±1 °C
Permeabilization
No
Specification
human decelluarized adipose matrix
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 29 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.05% typsin
Sample
Human Tissue sections (human whole fat tissue)
Specification
human whole fat tissue
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 05 2015

Answer

The epitope of ab23418 is located in domain V of Perlecan, which is from C-terminal amino acids 3663-4391 according to the uniprot file below:

http://www.uniprot.org/uniprot/P98160

Read More

Question
Answer

Thank you for calling Abcam earlier today.
I am sorry that you experienced issues with ab23418 in western blot. As we discussed I am sending you ab82346 as a free of charge replacement. Your new order number is *********
.
Please let me know if there is anything else I can help you with.

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Answer

Thank you very much for contacting us and for your patience. I apologise for the delay.

We have contacted the originator of the Anti-Heparan Sulfate Proteoglycan 2 antibody [A74]ab23418and enquired about epitope details. Unfortunately, there are no more details available than what is already stated on the datasheet: The epitope is located in domain V of Perlecan.

We unfortunately have only one Heparan Sulfate Proteoglycan 2 protein available in our catalog: ab114285 is a recombinant fragment of Human Heparan Sulfate Proteoglycan 2, amino acids 25-134.As thisrange of amino acids is different from domain V, ab114285 is therefore not a suitable option for your experimental setup.

We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion.Please do not hesitate to contact us again with other needs or with any questions about our products.

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Question
Answer

Code de remise pour ab86750 : ******
Code de remise pour ab23418 : ******
Date d’expiration pour ces deux codes : JJ MM AAAA
Je suis ravi que vous souhaitiez participer à notre offre et tester ab86750 et ab23418 en ICC/IF. Les codes de réduction ci-dessus sont utilisables sous 120 jours et pour l’achat de deux autres anticorps primaires.
Vous devez dans un premier temps soumettre une Abreview avec vos résultats ICC/IF en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab86750 et ab23418.
Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches.
Termes et Conditions : www.abcam.com/collaborationdiscount.

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Answer

Thank you for your inquiry. Unfortunately we have no information on any aa sequences for this antibody other than it is corneal endothelial cells from cow. Below is listed the following prices: Please let me know if I can be of further assistance.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Loading amount
50 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (10%SDS gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 08 2010

1-10 of 14 Abreviews or Q&A

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