Product nameAnti-Heparan Sulfate Proteoglycan 2 antibody [A74]
See all Heparan Sulfate Proteoglycan 2 primary antibodies
DescriptionMouse monoclonal [A74] to Heparan Sulfate Proteoglycan 2
Specificityab23418 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
Tested applicationsSuitable for: IHC-P, ICC/IF, WB, IP, IHC-Fr, ELISAmore details
Species reactivityReacts with: Cow, Human
Corneal endothelial cells (Cow).
EpitopeEpitope is located in domain V of Perlecan.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride
Concentration information loading...
PurityProtein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab23418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 22278369|
|ICC/IF||Use at an assay dependent concentration.|
|WB||1/100. Predicted molecular weight: 469 kDa.|
|IP||Use at an assay dependent concentration.|
|AP||Use at an assay dependent concentration. Can be used to separate recombinant domain I from full length perlecan.|
FunctionIntegral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.
Tissue specificityFound in the basement membranes.
Involvement in diseaseDefects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.
Sequence similaritiesContains 4 EGF-like domains.
Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
Contains 11 laminin EGF-like domains.
Contains 3 laminin G-like domains.
Contains 3 laminin IV type A domains.
Contains 4 LDL-receptor class A domains.
Contains 1 SEA domain.
modificationsProteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation.
Cellular localizationSecreted > extracellular space > extracellular matrix > basement membrane.
- Information by UniProt
- Basement membrane specific heparan sulfate proteoglycan core protein antibody
- Endorepellin (domain V region) antibody
- Heparan sulfate proteoglycan of basement membrane antibody
Immunohistochemical analysis of Human ovary tissue, staining Heparan Sulfate Proteoglycan 2 with ab23418.
Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/10 in diluent) for 30 minutes. An HRP-conjugated anti-mouse IgG was used as the secondary antibody.
ab23418 staining Heparan Sulfate Proteoglycan 2 in Human fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with methanol, permeabilized with 1% Triton X-100 and blocked with 5% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/600 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse monoclonal (1/200) was used as the secondary antibody.
This product has been referenced in:
- McGarrity S et al. Metabolic systems analysis of LPS induced endothelial dysfunction applied to sepsis patient stratification. Sci Rep 8:6811 (2018). WB ; Human . Read more (PubMed: 29717213) »
- Blache U et al. Notch-inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate. EMBO Rep 19:N/A (2018). Read more (PubMed: 29967223) »