Anti-Heparan Sulfate Proteoglycan 2 antibody [A76] (ab26265)


  • Product name
    Anti-Heparan Sulfate Proteoglycan 2 antibody [A76]
    See all Heparan Sulfate Proteoglycan 2 primary antibodies
  • Description
    Mouse monoclonal [A76] to Heparan Sulfate Proteoglycan 2
  • Host species
  • Specificity
    This antibody is highly specific for Heparan Sulfate Proteoglycan 2. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin) but it does cross-react with human thrombospondin.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Fr, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Sheep, Cow, Human
  • Immunogen

    Lysed bovine corneal endothelial cells and extracellular matrix.



Our Abpromise guarantee covers the use of ab26265 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/8000.
WB Use at an assay dependent concentration. Predicted molecular weight: 469 kDa.
IHC-Fr 1/100.
AP Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 18568676


  • Function
    Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
    Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
    The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.
  • Tissue specificity
    Found in the basement membranes.
  • Involvement in disease
    Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
    Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.
  • Sequence similarities
    Contains 4 EGF-like domains.
    Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 11 laminin EGF-like domains.
    Contains 3 laminin G-like domains.
    Contains 3 laminin IV type A domains.
    Contains 4 LDL-receptor class A domains.
    Contains 1 SEA domain.
  • Post-translational
    Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
    N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basement membrane specific heparan sulfate proteoglycan core protein antibody
    • Endorepellin (domain V region) antibody
    • Heparan sulfate proteoglycan of basement membrane antibody
    • HSPG 2 antibody
    • HSPG antibody
    • Hspg2 antibody
    • LG3 peptide antibody
    • Perlecan antibody
    • PGBM_HUMAN antibody
    • PLC antibody
    • Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) antibody
    • SJA antibody
    • SJS antibody
    • SJS1 antibody
    see all


This product has been referenced in:
  • Bird IM  et al. The skeletal phenotype of achondrogenesis type 1A is caused exclusively by cartilage defects. Development 145:N/A (2018). Read more (PubMed: 29180569) »
  • Rees MD  et al. Myeloperoxidase-derived oxidants selectively disrupt the protein core of the heparan sulfate proteoglycan perlecan. Matrix Biol 29:63-73 (2010). WB, ELISA ; Human . Read more (PubMed: 19788922) »

See all 4 Publications for this product

Customer reviews and Q&As

Gracias por contactarnos.

No hay protocolo concreto a seguir para llevar a cabo la recuperación antigénica de los tejidos previos al uso de este anticuerpo. En nuestra web hay unos protocolos generales muy útile...

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Thank you once again for your enquiries. I was in touch with our source for these antibodies again today, and I am pleased I now have the information for you.   1. Can ab26265 and ab23418 be used to detect for all kind of Heparan sulfate or are they...

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Vielen Dank für Ihren Anruf von vorher. Es tut mir leid zu hören, dass die Antikörper ab26265 und ab92611 immer noch nicht gute Resultate geben. Wie am Telefon besprochen, garantieren wir all unsere Produkte so zu funktionieren wie auf dem Datenblatt b...

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Thank you for your enquiry. Neither of the Abs that you have expressed an interest in have yet been tested for use in ICC. They have both been tested for IHC in fixed tissue sections. We do not appear to have an Ab to this target that has been specific...

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