• Product name
    Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74]
    See all Heparan Sulfate Proteoglycan 2/Perlecan primary antibodies
  • Description
    Mouse monoclonal [A74] to Heparan Sulfate Proteoglycan 2/Perlecan
  • Host species
  • Specificity
    ab23418 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, IP, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Tissue, cells or virus corresponding to Cow Heparan Sulfate Proteoglycan 2/Perlecan.

  • Epitope
    Epitope is located in domain V of Perlecan.
  • General notes

     This product was previously labelled as Heparan Sulfate Proteoglycan 2




Our Abpromise guarantee covers the use of ab23418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22278369
ICC/IF Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 469 kDa.
IP Use at an assay dependent concentration.
IHC-Fr 1/100.
ELISA 1/2000.
AP Use at an assay dependent concentration. Can be used to separate recombinant domain I from full length perlecan.


  • Function
    Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
    Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
    The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.
  • Tissue specificity
    Found in the basement membranes.
  • Involvement in disease
    Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
    Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.
  • Sequence similarities
    Contains 4 EGF-like domains.
    Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 11 laminin EGF-like domains.
    Contains 3 laminin G-like domains.
    Contains 3 laminin IV type A domains.
    Contains 4 LDL-receptor class A domains.
    Contains 1 SEA domain.
  • Post-translational
    Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
    N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basement membrane specific heparan sulfate proteoglycan core protein antibody
    • Endorepellin (domain V region) antibody
    • Heparan sulfate proteoglycan of basement membrane antibody
    • HSPG 2 antibody
    • HSPG antibody
    • Hspg2 antibody
    • LG3 peptide antibody
    • Perlecan antibody
    • PGBM_HUMAN antibody
    • PLC antibody
    • Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) antibody
    • SJA antibody
    • SJS antibody
    • SJS1 antibody
    see all


  • Immunohistochemical analysis of Human ovary tissue, staining Heparan Sulfate Proteoglycan 2/Perlecan with ab23418.

    Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/10 in diluent) for 30 minutes. An HRP-conjugated anti-mouse IgG was used as the secondary antibody.

    See Abreview

  • ab23418 staining Heparan Sulfate Proteoglycan 2/Perlecan in Human fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with methanol, permeabilized with 1% Triton X-100 and blocked with 5% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/600 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse monoclonal (1/200) was used as the secondary antibody.

    See Abreview


This product has been referenced in:
  • McGarrity S  et al. Metabolic systems analysis of LPS induced endothelial dysfunction applied to sepsis patient stratification. Sci Rep 8:6811 (2018). WB ; Human . Read more (PubMed: 29717213) »
  • Blache U  et al. Notch-inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate. EMBO Rep 19:N/A (2018). Read more (PubMed: 29967223) »
See all 9 Publications for this product

Customer reviews and Q&As

11-14 of 14 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Ovary)
Antigen retrieval step
Heat mediated
Blocking step
No blocking step used for 1 minute(s) · Concentration: 0

Mr. Rudolf Jung

Verified customer

Submitted Feb 20 2007


Thank you for your enquiry. Regarding ab23418 (Heparan Sulfate Proteoglycan 2 antibody [CSI 001-74]), this antibody has been used on paraffin-embedded tissues in a paper that can be found at: http://ops.abcam.com/admin/AbcamShare/crm/attachments/Melrose%20J%20Histochem%20Cell%20Biol%202002%20CSI%20001-71,%20CSI%20001-74%20og%20CSI%20001-76.pdf The IHC protocol for perlecan is at the bottom of page 270, which used a fixative named Histochoice, which does not have formaldehyde in it. http://www.emsdiasum.com/microscopy/products/histology/fixatives.aspx Therefore, it is not clear if this antibody will work on formalin-fixed tissues. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for your enquiry. Neither of the Abs that you have expressed an interest in have yet been tested for use in ICC. They have both been tested for IHC in fixed tissue sections. We do not appear to have an Ab to this target that has been specifically tested on cells. There are several resources that I would suggest: 1) Biocompare.com- a website that will allow you to search for Abs from a variety of companies. 2) The website “IHC world”: http://www.ihcworld.com/protocol_database.htm Despite the fact that this web site is focused on IHC and not ICC there is a lot of useful information that is transferable. 3) Abcam has an ICC protocol, which is accessible from the public homepage under the "protocols" hyper link, this will offer information on staining procedures e.t.c... Should you require any further assistance then please do not hesitate to get back in touch with me.

Read More
Immunocytochemistry/ Immunofluorescence
Human Cell (Human fibroblast)
Yes - triton X-100 1%
Human fibroblast
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jun 28 2012

11-14 of 14 Abreviews or Q&A

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