Overview

  • Product name
    Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74]
    See all Heparan Sulfate Proteoglycan 2/Perlecan primary antibodies
  • Description
    Mouse monoclonal [A74] to Heparan Sulfate Proteoglycan 2/Perlecan
  • Host species
    Mouse
  • Specificity
    ab23418 is highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, IP, IHC-Fr, ELISAmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Tissue, cells or virus corresponding to Cow Heparan Sulfate Proteoglycan 2/Perlecan.

  • Epitope
    Epitope is located in domain V of Perlecan.
  • General notes

     This product was previously labelled as Heparan Sulfate Proteoglycan 2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab23418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 22278369
ICC/IF Use at an assay dependent concentration.
WB 1/100. Predicted molecular weight: 469 kDa.
IP Use at an assay dependent concentration.
IHC-Fr 1/100.
ELISA 1/2000.
AP Use at an assay dependent concentration. Can be used to separate recombinant domain I from full length perlecan.

Target

  • Function
    Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
    Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
    The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity.
  • Tissue specificity
    Found in the basement membranes.
  • Involvement in disease
    Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
    Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage.
  • Sequence similarities
    Contains 4 EGF-like domains.
    Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 11 laminin EGF-like domains.
    Contains 3 laminin G-like domains.
    Contains 3 laminin IV type A domains.
    Contains 4 LDL-receptor class A domains.
    Contains 1 SEA domain.
  • Post-translational
    modifications
    Proteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
    N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > basement membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Basement membrane specific heparan sulfate proteoglycan core protein antibody
    • Endorepellin (domain V region) antibody
    • Heparan sulfate proteoglycan of basement membrane antibody
    • HSPG 2 antibody
    • HSPG antibody
    • Hspg2 antibody
    • LG3 peptide antibody
    • Perlecan antibody
    • PGBM_HUMAN antibody
    • PLC antibody
    • Schwartz Jampel syndrome 1 (chondrodystrophic myotonia) antibody
    • SJA antibody
    • SJS antibody
    • SJS1 antibody
    see all

Images

  • Immunohistochemical analysis of Human ovary tissue, staining Heparan Sulfate Proteoglycan 2/Perlecan with ab23418.

    Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/10 in diluent) for 30 minutes. An HRP-conjugated anti-mouse IgG was used as the secondary antibody.

    See Abreview

  • ab23418 staining Heparan Sulfate Proteoglycan 2/Perlecan in Human fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with methanol, permeabilized with 1% Triton X-100 and blocked with 5% BSA for 20 minutes at 22°C. Samples were incubated with primary antibody (1/600 in diluent) for 12 hours at 4°C. An AlexaFluor®488-conjugated goat anti-mouse monoclonal (1/200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • McGarrity S  et al. Metabolic systems analysis of LPS induced endothelial dysfunction applied to sepsis patient stratification. Sci Rep 8:6811 (2018). WB ; Human . Read more (PubMed: 29717213) »
  • Blache U  et al. Notch-inducing hydrogels reveal a perivascular switch of mesenchymal stem cell fate. EMBO Rep 19:N/A (2018). Read more (PubMed: 29967223) »
See all 9 Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews

Application
Flow Cytometry
Sample
Rhesus monkey Cell (chorioretinal endothelial cell)
Permeabilization
No
Gating Strategy
size and granularity
Specification
chorioretinal endothelial cell
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with Accutase
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde

Andrea Cabrera

Verified customer

Submitted Sep 22 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human decelluarized adipose matrix)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate buffer pH 6.0 for 40 minutes at 97.0 ±1 °C
Permeabilization
No
Specification
human decelluarized adipose matrix
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 29 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human whole fat tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.05% typsin
Permeabilization
No
Specification
human whole fat tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Mar 05 2015

Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (10%SDS gel)
Loading amount
50 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 08 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ovary)
Specification
Ovary
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
No blocking step used for 1 minute(s) · Concentration: 0

Mr. Rudolf Jung

Verified customer

Submitted Feb 20 2007

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human fibroblast)
Permeabilization
Yes - triton X-100 1%
Specification
Human fibroblast
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Methanol

Abcam user community

Verified customer

Submitted Jun 28 2012

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