Question (27604) | Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74] (ab23418)

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Question

Dear Sir/Madam, We are working on a laboratory located on Viet Nam and looking at Human Heparan Sulfate Measurements in dengue patient plasma. In the past, we ordered the Heparan Sulfate ELISA Kits from Seikagaku Company (Japan) to do researches related to Heparan Sulfate, these kits worked well for our experiments. However, these kits are no longer produced anymore. So, we are planning to design an ELISA protocol by ourselves for the researches in future. We found that your company has some monoclonal antibodies could be used for our experiments. We would like to ask you some information about these products. Could you please help us for the information? The products named anti –Heparan Sulfate Proteoglycan 2 antibody –A76 (ab26265) and anti-Heparan Sulfate Proteoglycan 2 antibody- A74 (ab23418) that could be used to detect for all kind of Heparan sulfate or only specific to HS Proteoglycan 2? What kind of the monoclonal antibody (ab 266265 or ab 23418) is more specific to HS if we plan to use these antibodies for coating the plates? What is the Epitope of ab 26265 antibody? It doesn’t be shown in the product instruction. The product called anti-Heparan sulfate Proteoglycan 2 antibody –A7L6 (Biotin) (ab79465) could be used for the ELISA test? This application doesn’t be suggested in the product instruction. The antibody ab79465 (Biotin) could be used as a biotinylated antibody for the ELISA plates pre- coated ab26265 or ab23418 , then it will be detected by HRP-Conjugated Streptavidin? I am looking forward to your reply as soon as possible. Please have a look. Thank you very much.  

Answer

Thank you once again for your enquiries. I was in touch with our source for these antibodies again today, and I am pleased I now have the information for you.   1. Can ab26265 and ab23418 be used to detect for all kind of Heparan sulfate or are they only specific to HS Proteoglycan 2? As far as we are aware. these antibodies are specific to HS Proteoglycan 2, but this has not been tested in the laboratory. If the epitope is in one of the domains that exists in the other heparan sulfates it might detect these,  however I am sorry the epitope has not been mapped. 2. Which is more specific to HS if we plan to use these antibodies for coating plates in ELISA? The product specification states:ab26265 is more sensitive than ab23418 in ELISA. 3. What is the Epitope of ab26265 antibody? I am sorry the epitope has not been mapped. 4. ab79465 could be used for the ELISA test? I am sorry to confirm this antibody has not been tested in ELISA.  All tested and guaranteed species and applications are listed on the online datasheet and updated as soon as we receive new information. If you would like to test this antibody in ELISA, please letme know before you purchase as you may be eligable for a testing discount. 5. I am sorry to confirm these antibodies  have not been tested together in sandwich ELISA (sELISA) and are not guaranteed for this application.  ab26265 and ab23418 have been tested in indirect or direct ELISA only. I hope this information will be helpful to you. Thank you for your patience, and if you have any further questions, or if you would like to test any of these antibodies in an untestd appcliation,  please do not hesitate to contact me.  

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