Western blot abreview for Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74]

Good
Abreviews
Application
Western blot
Sample
Human Tissue lysate - whole (brain)
Gel Running Conditions
Reduced Denaturing (10%SDS gel)
Loading amount
50 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 5% milk
Dilution
1/100

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: rabbit anti mouse IgG antibody
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 85 kDa

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Submitted Jul 08 2010

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