Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human decelluarized adipose matrix)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate buffer pH 6.0 for 40 minutes at 97.0 ±1 °C
Permeabilization
No
Specification
human decelluarized adipose matrix
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Dilution
1/100
Name
Non-Abcam antibody was used: goat anti-mouse secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

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Submitted Jul 29 2015

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