Flow Cytometry abreview for Anti-Heparan Sulfate Proteoglycan 2/Perlecan antibody [A74]

Good
Abreviews
Application
Flow Cytometry
Sample
Rhesus monkey Cell (chorioretinal endothelial cell)
Permeabilization
No
Gating Strategy
size and granularity
Specification
chorioretinal endothelial cell
Preparation
Cell harvesting/tissue preparation method: cells were detached from tissue culture plates with Accutase
Sample buffer: 2mM EDTA + 0.5% BSA in PBS
Fixation
Paraformaldehyde

Other product details

Incubation time
20 minute(s) · Temperature: 4°C · Diluent: 2mM EDTA + 0.5% BSA in PBS
Dilution
1/100

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Vector Labs R-Phycoerythrin Horse Anti-Mouse
Host species: Mouse
Clonality: Monoclonal
Conjugation: Phycoerythrin

Additional data

Abcam response
Thank you for taking part in our AbTrial program and your valuable feedback in Flow Cytometry and Rhesus Monkey. Both species and application have not been tested before. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial program.

Andrea Cabrera

Verified customer

Submitted Sep 22 2017

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