Key features and details
- Rabbit polyclonal to Heparanase 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Heparanase 1 antibody
See all Heparanase 1 primary antibodies
DescriptionRabbit polyclonal to Heparanase 1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human spleen tissue. WB: HeLa and HepG2 cell lysates; Recombinant human Heparanase 1 protein.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232817 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 61 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionEndoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, it is considered to be a potential target for anti-cancer therapies.
Tissue specificityHighly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues.
Sequence similaritiesBelongs to the glycosyl hydrolase 79 family.
modificationsProteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, 8 kDa and 50 kDa product. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility.
Cellular localizationLysosome membrane. Secreted. Secreted, internalised and transferred to late endosomes/lysosomes as a proheparanase. In lysosomes, it is processed into the active form, the heparanase. The uptake or internalisation of proheparanase is mediated by HSPGs. Heparin appears to be a competitor and retain proheparanase in the extracellular medium.
- Information by UniProt
- Endo glucoronidase antibody
- Endo-glucoronidase antibody
- HEP antibody
Formalin-fixed, paraffin-embedded human spleen tissue stained for Heparanase 1 using ab232817 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Heparanase 1 antibody (ab232817) at 3 µg/ml + Recombinant human Heparanase 1 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 61 kDa
All lanes : Anti-Heparanase 1 antibody (ab232817) at 3 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : Human liver lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 61 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232817 has not yet been referenced specifically in any publications.