Overview

  • Product name

    Heparin Sepharose®
  • Product overview

    High binding capacity (>0.4mg/mL). Minimal leaching of ligand. For column or batch purification of heparin-binding proteins & cation exchange (ab193268).


    Contents:


    Supplied as a 50% slurry in 20 % Ethanol; >2.5 mg heparin per mL Sepharose® beads.


     


    Features:


    Heparin beads have been widely used in affinity purification of various heparin-binding proteins or ligands, such as antithrombin III, lipoprotein, as well as DNA binding proteins (transcription factors, virus coat proteins etc). Abcam’s Heparin Sepharose® is designed for purification of heparin-binding proteins and ligands. It can also be used as a high capacity cation exchange medium. Specific proteins can be separated by using different concentrations of salt or a salt gradient. This Heparin Sepharose® formulation exhibits excellent binding capacity, high flow rate, no significant loss of the heparin ligand and a pH stability range of 2-10.


    These beads are for use in column purification. If used in batch purification, we recommend not exceeding 150 x g when centrifuging.


    Store beads at 4°C.


    The beads may be damaged above 40°C.


    DO NOT FREEZE.


    Wash beads 3 times with 3x bead volume of desired buffer before use.


     


    Applications:


    Purification of heparin-binding proteins, enzymes or other ligands.


     


    Sepharose is a registered trademark of GE Healthcare

  • Notes

    Heparin Sepharose® is prepared by covalently coupling heparin to epoxy-activated 6% cross-linked Sepharose® beads. The coupling was optimized to give a high binding capacity and could be greater than 0.4 mg of heparin-binding protein (such as thrombin) per ml of wet gel.

  • Tested applications

    Suitable for: Purificationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab193268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Purification Use at an assay dependent concentration.

Purification of heparin-binding proteins, enzymes or other ligands.

References

ab193268 has not yet been referenced specifically in any publications.

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