Anti-Hepatitis B Virus Core Antigen antibody [10E11] (ab8639)

Submit an Abreview Q&A (15)Specific References (3)

Overview

  • Product name
    Anti-Hepatitis B Virus Core Antigen antibody [10E11]
    See all Hepatitis B Virus Core Antigen primary antibodies
  • Description
    Mouse monoclonal [10E11] to Hepatitis B Virus Core Antigen
  • Host species
    Mouse
  • Specificity
    This antibody reacts with HBV Core Antigen (amino acid residues 1-10). Ab8639 should recognize both the precoreprotein and core protein.
  • Tested applications
    Suitable for: ICC/IF, WB, IP, ELISA, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Hepatitis B virus
  • Immunogen

    Purified Denatured Hepatitis B Core Antigen

  • Epitope
    aa positions 1-10

Properties

Applications

Our Abpromise guarantee covers the use of ab8639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P 1/100.
IHC-Fr 1/100.

Target

  • Relevance
    Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
  • Cellular localization
    Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
  • Database links
    • Alternative names
      • C antibody
      • Capsid protein antibody
      • Core and e antigen antibody
      • core antibody
      • Core antigen antibody
      • Core protein antibody
      • HBc antibody
      • HBcAg antibody
      • HBVgp4 antibody
      • Hepatitis B core antigen antibody
      • Hepatitis B Virus core antigen antibody
      • p21.5 antibody
      • precore/core protein antibody
      see all

    References

    This product has been referenced in:
    • Peyret H  et al. Tandem fusion of hepatitis B core antigen allows assembly of virus-like particles in bacteria and plants with enhanced capacity to accommodate foreign proteins. PLoS One 10:e0120751 (2015). WB . Read more (PubMed: 25830365) »
    • Macdonald DC  et al. Harnessing alveolar macrophages for sustained mucosal T-cell recall confers long-term protection to mice against lethal influenza challenge without clinical disease. Mucosal Immunol N/A:N/A (2013). Read more (PubMed: 23715172) »

    See all 3 Publications for this product

    Publishing research using ab8639? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    15 reviews or Q&A

    10
    Votes: Highest First

    Thank you and I appreciate you taking some care over this.
    Obviously, if you look at our account, you will see we use a huge amount of this. A substantial change in titre would make this very expensive indeed and we would need some form of recompe...

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    Thank you very for your cooperation in this case. I apologize for being late as our collaborator lab was unable to get back to me in time.

    At Abcam we have a strict ethical policies, we care about animals and always track if our collaborator ...

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    I am not really sure that this is sorting the problem at all. Rather than just take pot luck, would it not be sensible to send us a small aliquot of whatever batches you have to test first?
    Changing the antibody is completely out of the question; t...

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    We unfortunately do not keep small samples of antibodies so we are unable to send you sample of each lot. The only thing we can do is to ship one vial of new lot.

    Let me know if you are interested in receivingone free of charge vial.

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    I'm afraid the discount is somewhat missing the point. What we really want is for the antibody to be concentrated prior to sale. The new batch is massively less effective than the tissue culture supernatant. Obviously, XXXXXXXXX is welcome, but it does...

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    Thank you very much for your patience. I apologize for the delay as it took me longer than expected, due to interdepartmental communications.

    I have checked the previous history of this antibody and found that the purity of this antibody was a...

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    Thank you and I appreciate you taking some care over this.
    Obviously, if you look at our account, you will see we use a huge amount of this. A substantial change in titre would make this very expensive indeed and we would need some form of recompe...

    Read More

    I am currently having discussions with lab. I will get back to you soon.

    Many thanks for having patience!

    Thanks for this.
    The most obvious question is why is the antibody the same price when it is clearly at a significantly lower titre?

    Regards

    Thank you for contacting us.

    We unfortunately do not know the concentration and titre of the product because it is not a purified antibody. Generally the titre of tissue culture supernatant is lower than the ascites, which was the basis of m...

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    please find attached completed questionnaire and collated results (in ppt file) with regard to the decreased efficiency of the anti-HBV core antigen monoclonal antibody [10E11] (product code: ab8639; lot: GR17742-1), which we have routinely bought from...

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    Thank you very much for your email and for filling the questionnaire. I am very sorry to hear about the problems you are experiencing.

    The most significant change happened between the lot #637899 and lot #GR17742-1 is the purity of the antibo...

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    The lot 637899 works well in western blot however GR17742-1 produces very faint band.
    - dilution 1/300
    - Same protocol used between different lots

    Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    As we discussed on the ...

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    1. Order details: * Batch number: 140202 * Abcam order or Purchase order number: ab8639 * Antibody storage conditions (temperature/reconstitution etc) Working antibody store at 4?, Aliquot and store at -80? 2. Please describe the problem (...

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    Thank you for providing extensive details of the customer's protocol, as well as images, it enabled me to assess very rapidly that there was a problem with the vial of ab8639 that you received. I can offer a replacement vial or credit note, whicheve...

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    What is the immunogen used to generate ab8638,ab 8639, ab8637, it is meant to recognise the core protein of Hepatitis B, can we confirm this is the core and NOT the precore protein. The customer wants to detect the core protein of Hep B, in particular...

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    Ab8637 and ab8639 should recognize the precorerotein in WB but there is no "cross-reaction" as all proteins are distinguishable by mobility on the gel (i.e they will recognise both the precoreprotein and core protein). AB8638 may or may not recogni...

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    Does this antibody recognize the HBsAg subtype adw?

    Thank you for your enquiry. We don not have information as to whether it recognize the HBsAg subtype adw. Ab8639 reacts with HBV Core Antigen (amino acid residues 1-10) and this antibody has been characterized in great detail in the following paper: ...

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