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please find attached completed questionnaire and collated results (in ppt file) with regard to the decreased efficiency of the anti-HBV core antigen monoclonal antibody [10E11] (product code: ab8639; lot: GR17742-1), which we have routinely bought from you with variable results. Upon investigation it is now apparent that the problem goes back to earlier experiments where we have used the same lot number. The good results have been whenever we have gone to frozen stocks of an earlier batch (lot: 637899)
We rely upon this antibody for routine immunoassays and are therefore very keen to learn why there has been such a marked reduction in apparent titre in the recent batch.
Asked on May 14 2012
Thank you very much for your email and for filling the questionnaire. I am very sorry to hear about the problems you are experiencing.
The most significant change happened between the lot #637899 and lot #GR17742-1 is the purity of the antibody. The lot #637899 was produced as ascites and the lot GR17742 was produced as tissue culture supernatant. The titre (concentration) of antibodies in ascites is always higher than the tissue culture supernatants, this may explain the difference in results.
The datasheet of this antibody was changed in February this year; reflecting the correct information about the product (purity). I suppose this info might have missed when ordering the recent lot.
To overcome this change we always suggest our customer changing the dilution factor. Have you tried any troubleshooting with the current lot?
I hope this information will be useful. I will look forward to hearing from you soon for further assistance.
Answered on May 14 2012