Anti-Hepatitis B Virus Core Antigen antibody [14E11] (ab8638)

Submit an Abreview Q&A (12)Specific References (5)

Overview

  • Product name
    Anti-Hepatitis B Virus Core Antigen antibody [14E11]
    See all Hepatitis B Virus Core Antigen primary antibodies
  • Description
    Mouse monoclonal [14E11] to Hepatitis B Virus Core Antigen
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, IHC-FoFr, IHC-Fr, ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Hepatitis B virus
  • Immunogen

    Purified Denatured Hepatitis B Core Antigen

  • Epitope
    135-141aa (PNAPILS)

Properties

Applications

Our Abpromise guarantee covers the use of ab8638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-FoFr 1/100.
IHC-Fr 1/100.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-P 1/100.
WB 1/1000.

Target

  • Relevance
    Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
  • Cellular localization
    Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
  • Database links
    • Alternative names
      • C antibody
      • Capsid protein antibody
      • Core and e antigen antibody
      • core antibody
      • Core antigen antibody
      • Core protein antibody
      • HBc antibody
      • HBcAg antibody
      • HBVgp4 antibody
      • Hepatitis B core antigen antibody
      • Hepatitis B Virus core antigen antibody
      • p21.5 antibody
      • precore/core protein antibody
      see all

    References

    This product has been referenced in:
    • Liang P  et al. Efficient Humoral and Cellular Immune Responses Induced by a Chimeric Virus-like Particle Displaying the Epitope of EV71 without Adjuvant. Biomed Environ Sci 31:343-350 (2018). WB ; Hepatitis B virus . Read more (PubMed: 29866216) »
    • Ye X  et al. Chimeric virus-like particle vaccines displaying conserved enterovirus 71 epitopes elicit protective neutralizing antibodies in mice through divergent mechanisms. J Virol 88:72-81 (2014). Read more (PubMed: 24131712) »

    See all 5 Publications for this product

    Publishing research using ab8638? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    12 reviews or Q&A

    10
    Votes: Highest First

    We had just spoken over the telephone regarding 3 antibodies (ab8638, ab8639 and ab18684) directed against the HBV core. They have been validated in Western blots, but we would be using them also for immunogold staining. Is there any chance that we cou...

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    We unfortunately do not sell starter packs so I am sorry we cannot send the same. These products are stored in standard amount and the information is available on the datasheets.

    Hi, I have previously ordered this mouse monoclonal antibody to HBcAg (ab8638) several times before, and the accompanying datasheet (attached) described the epitope as amino acids 130-140. Having looked at your website this morning for the same a...

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    Thank for your inquiry. I would like to reassure you that the antibody is the same as previously. There is no batch to batch variability of the immunogen sequence in monoclonal antibodies. This a monoclonal antibody was known to bind to the HBV...

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    I would like to get some information about the product mouse monoclonal [14E11] to hepatitis B virus core antigen (ab8638). 1. It detects the amino acid 130-140, may I know that what is the sequence for it? 2. How much total lysate from the mammal...

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    1. The epitope is 135-141 (PNAPILS) 2. This depends on how much virus or core antigen is in the cells. You can load different amounts in parallel. For example 5ug (microgram) and 20ug. 3. Example version of Laemmli 2X buffer 4% SDS 10% 2-me...

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    Dear Sir, It seems that the band I find is the aggregates of the 21kDa Hepatitis B virus Core Antigen. The most important thing for me is to know how to isolate the aggregates to be monomer. Best regards,

    Thank you for your enquiry. Further to discussions with the lab I have determined that the approach that you are using (n)on-reducing gel at 12%) is not the best system to isolate monomeric units of HBcAg core particles. To dissolve HBcAg core part...

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    BATCH NUMBER 143129 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific band, it shows the wrong band size between 120 to 180kd. SAMPLE Species is from HepG2 2.2.15 cell. Cell extract contains NaCl 150 mM,NP-...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I am surprised by the size of the band that you have been detecting. I am in correspondence with the source of this antibody to determine the ...

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    What is the immunogen used to generate ab8638,ab 8639, ab8637, it is meant to recognise the core protein of Hepatitis B, can we confirm this is the core and NOT the precore protein. The customer wants to detect the core protein of Hep B, in particular...

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    Ab8637 and ab8639 should recognize the precorerotein in WB but there is no "cross-reaction" as all proteins are distinguishable by mobility on the gel (i.e they will recognise both the precoreprotein and core protein). AB8638 may or may not recogni...

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    thanks for the information. Is there any information that you have regarding recommended starting concentrations for use of these antibodies as either capture or detection antibodies?

    Here is the information that I could obtain regarding ab8639, ab8638, and ab8637. The recommended starting dilutions are 1:1,000 and down in 10-fold increments. As for detection, any known detection method would work. Once again, those mabs had being c...

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    thanks for the information. Is there any information that you have regarding recommended starting concentrations for use of these antibodies as either capture or detection antibodies?

    I have received the following information regarding ab8255 and ab2045, but will have to get back to you about the other antibodies. The originator has said that they tested the antibodies as pairs in internal ELISA. Possible pairs are H6F5 (ab2045) - H...

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    Customer would like more details about how these antibodies were tested for application in ELISA.

    Thank you for your enquiry. I was able to obtain the following information regarding the use of these antibodies in ELISA. Ab8255 and Ab2045: These antibodies have been tested in ELISA with the native antigen. The antibodies can be used as a pair in...

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    Thanks for your rapid reply. I have one more question. My end user wants to know the type of HBV core antigen.(i.e.ayw1, ayw2...) He is supposed to it's type is ayw. Inform me about that, if you can confirm it. Best Regareds

    Thank you for your reply. The only data we have regarding this is that it is an ayw variant as described in: Bichko et. al. Subtype ayw variant of hepatitis B virus. DNA primary structure analysis. FEBS Lett. 1985 Jun 3;185(1):208-12.

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