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  1. Link

    hepatitis-b-virus-core-antigen-antibody-14e11-ab8638.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus Hepatitis B
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Anti-Hepatitis B Virus Core Antigen antibody [14E11] (ab8638)

  • Datasheet
Submit a review Q&A (12)References (12)

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Promotion Information

Abpromise

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Find out more.

Key features and details

  • Mouse monoclonal [14E11] to Hepatitis B Virus Core Antigen
  • Suitable for: ELISA, IHC-FoFr, IHC-Fr, ICC/IF, IP, IHC-P, WB
  • Reacts with: Hepatitis B virus
  • Isotype: IgG2b

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Secondary
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Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Protein
Recombinant Hepatitis B Virus Core Antigen protein (ab49013)

View more associated products

Overview

  • Product name

    Anti-Hepatitis B Virus Core Antigen antibody [14E11]
    See all Hepatitis B Virus Core Antigen primary antibodies
  • Description

    Mouse monoclonal [14E11] to Hepatitis B Virus Core Antigen
  • Host species

    Mouse
  • Specificity

    ab8638 was raised against serotype ayw but will work with all other genotypes. 

  • Tested applications

    Suitable for: ELISA, IHC-FoFr, IHC-Fr, ICC/IF, IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Hepatitis B virus
  • Immunogen

    Tissue, cells or virus corresponding to Hepatitis B Virus Core Antigen. Purified Denatured Hepatitis B Core Antigen

  • Epitope

    135-141aa (PNAPILS)
  • General notes

    This product is raised in the same, but denatured, HBcAg protein sequence as ab8637, hence have more robust activity in denaturing western blots/IF etc.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Bioreactor harvest, serum-free medium, no protein added
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    14E11
  • Myeloma

    Sp2/0
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Hepatitis B
    • Cancer
    • Oncoproteins/suppressors
    • Viral proteins
    • Hepatitis B

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Hepatitis B Virus Core Antigen protein (ab49013)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
IHC-FoFr
1/100.
IHC-Fr
1/100.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
1/100.
WB
1/1000.
Notes
ELISA
Use at an assay dependent concentration.
IHC-FoFr
1/100.
IHC-Fr
1/100.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
1/100.
WB
1/1000.

Target

  • Relevance

    Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
  • Cellular localization

    Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
  • Database links

    • Entrez Gene: 2828293 Hepatitis B virus
    • Entrez Gene: 944568 Hepatitis B virus
    • SwissProt: P03146 Hepatitis B virus
    • SwissProt: P03147 Hepatitis B virus
    • SwissProt: P03148 Hepatitis B virus
    • SwissProt: P0C573 Hepatitis B virus
    • SwissProt: P69706 Hepatitis B virus
    • Alternative names

      • C antibody
      • Capsid protein antibody
      • Core and e antigen antibody
      • core antibody
      • Core antigen antibody
      • Core protein antibody
      • HBc antibody
      • HBcAg antibody
      • HBVgp4 antibody
      • Hepatitis B core antigen antibody
      • Hepatitis B Virus core antigen antibody
      • p21.5 antibody
      • precore/core protein antibody
      see all

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (12)

    Publishing research using ab8638? Please let us know so that we can cite the reference in this datasheet.

    ab8638 has been referenced in 12 publications.

    • Colant N  et al. Escherichia Coli-Based Cell-Free Protein Synthesis for Iterative Design of Tandem-Core Virus-Like Particles. Vaccines (Basel) 9:N/A (2021). PubMed: 33669126
    • Lucifora J  et al. Evidence for long-term association of virion-delivered HBV core protein with cccDNA independently of viral protein production. JHEP Rep 3:100330 (2021). PubMed: 34409278
    • Ma A  et al. A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B. Hum Vaccin Immunother 16:756-778 (2020). PubMed: 31687879
    • Xie H  et al. ROS/NF-?B Signaling Pathway-Mediated Transcriptional Activation of TRIM37 Promotes HBV-Associated Hepatic Fibrosis. Mol Ther Nucleic Acids 22:114-123 (2020). PubMed: 32916597
    • Song C  et al. Associations Between Hepatitis B Virus Infection and Risk of All Cancer Types. JAMA Netw Open 2:e195718 (2019). PubMed: 31199446
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 12 Abreviews or Q&A

    Question

    We had just spoken over the telephone regarding 3 antibodies (ab8638, ab8639 and ab18684) directed against the HBV core. They have been validated in Western blots, but we would be using them also for immunogold staining. Is there any chance that we could have a starter pack of the three antibodies so that we can ascertain which one of them would be most optimal for immunogold staining, please?

    Please do not hesitate to contact me should you need any further information.

    With best wishes,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 07 2014

    Answer

    We unfortunately do not sell starter packs so I am sorry we cannot send the same. These products are stored in standard amount and the information is available on the datasheets.

    Read More

    Padamjeet Singh

    Abcam Scientific Support

    Answered on Jan 07 2014

    Question

    Hi, I have previously ordered this mouse monoclonal antibody to HBcAg (ab8638) several times before, and the accompanying datasheet (attached) described the epitope as amino acids 130-140. Having looked at your website this morning for the same antibody, the epitope is now listed as aa 135-141. Although I appreciate this is in the same region, I need to know whether the epitope has changed as a result of a new batch of antibody or whether it has just been reclassified, and the antibody from the old datasheet and the new datasheet are in fact the same. Many thanks,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 05 2011

    Answer

    Thank for your inquiry. I would like to reassure you that the antibody is the same as previously. There is no batch to batch variability of the immunogen sequence in monoclonal antibodies. This a monoclonal antibody was known to bind to the HBV Core Antigen which corresponds to the area of amino acid residues 130-140. More recently we discovered that the epitope had been mapped and the literature indicates that this clone (14E11) recognizes the amino acids 135-141 which corresponds to the following sequence: PNAPILS. I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

    Read More

    Abcam Scientific Support

    Answered on Aug 05 2011

    Question

    I would like to get some information about the product mouse monoclonal [14E11] to hepatitis B virus core antigen (ab8638). 1. It detects the amino acid 130-140, may I know that what is the sequence for it? 2. How much total lysate from the mammalia cells should I load for the western blot? 3. What is the Laemmli buffer you use to denature the sample?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 26 2011

    Answer

    1. The epitope is 135-141 (PNAPILS) 2. This depends on how much virus or core antigen is in the cells. You can load different amounts in parallel. For example 5ug (microgram) and 20ug. 3. Example version of Laemmli 2X buffer 4% SDS 10% 2-mercaptoehtanol 20% glycerol 0.004% bromophenol blue 0.125 M Tris HCl Check the pH and bring it to pH 6.8. Our online protocols are at www.abcam.com/protocols I highly recommend to look at the publications: PubMed: 19327810 and 16378961.

    Read More

    Abcam Scientific Support

    Answered on Jan 26 2011

    Question

    Dear Sir, It seems that the band I find is the aggregates of the 21kDa Hepatitis B virus Core Antigen. The most important thing for me is to know how to isolate the aggregates to be monomer. Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 04 2006

    Answer

    Thank you for your enquiry. Further to discussions with the lab I have determined that the approach that you are using (n)on-reducing gel at 12%) is not the best system to isolate monomeric units of HBcAg core particles. To dissolve HBcAg core particles to monomers, the sample should be dissolved in the Laemmli buffer with 5%SDS, 5% Mercaptoethanol (added fresh each time), and boiled for 10 min on a water bath. Should you continue to get what you expect to be polymers of this protein please do not hesitate to get back in touch with me.

    Read More

    Abcam Scientific Support

    Answered on Jul 10 2006

    Question

    BATCH NUMBER 143129 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Non-specific band, it shows the wrong band size between 120 to 180kd. SAMPLE Species is from HepG2 2.2.15 cell. Cell extract contains NaCl 150 mM,NP-40 1.0%,Nadeoxycholate 0.5% and SDS 0.1%. PRIMARY ANTIBODY with ab8638(ABCAM) at 1:400 in TBST with 5% defated mile for about 4 hours at room temprature. Washed with TBST three times. DETECTION METHOD ECL kit from pierce POSITIVE AND NEGATIVE CONTROLS USED use GepG2 cell as the negative control and hepatitis b virus core antigen as positive control ANTIBODY STORAGE CONDITIONS store at -20?C SAMPLE PREPARATION The Buffer contains 4?Stacking Buffer, SDS, Glycerol, mercaptoethanol and Bromophenol blue. AMOUNT OF PROTEIN LOADED 50ug ELECTROPHORESIS/GEL CONDITIONS Non-reducing gel at 12% TRANSFER AND BLOCKING CONDITIONS wet transfer and blocking with 5% defated milk for 1 hour SECONDARY ANTIBODY with the goat anti mouse antibody from [another company] at 1:3000 incubation for 2 hours. Then washed three times. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES I have find the strand between 120 to 180kd. But it should at near 21kd.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 27 2006

    Answer

    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I am surprised by the size of the band that you have been detecting. I am in correspondence with the source of this antibody to determine the expected size of the band using this antibody. At this stage I would like to recommend that you transfer the protein lysate that you have prepared and cut the blot using a scalpel so that only proteins of a molecular weight less than 100KDa are immunoblotted and the antibody is concentrated over proteins of appropriate size. I would like to recommend that an overnight incubation is performed using 3% BSA as a blocking agent. This approach should significantly increase the chances of detecting the Hepatitis core antigen. I hope this information helps. Should this approach not improve your results please do not hesitate to get back in touch with me.

    Read More

    Abcam Scientific Support

    Answered on Jun 29 2006

    Question

    What is the immunogen used to generate ab8638,ab 8639, ab8637, it is meant to recognise the core protein of Hepatitis B, can we confirm this is the core and NOT the precore protein. The customer wants to detect the core protein of Hep B, in particular fragment 1-150 (N term) in WB.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2005

    Answer

    Ab8637 and ab8639 should recognize the precorerotein in WB but there is no "cross-reaction" as all proteins are distinguishable by mobility on the gel (i.e they will recognise both the precoreprotein and core protein). AB8638 may or may not recognize your protein fragment aa1-150, since its epitope is aa aa35-140 of the core protein, which is really close to the N terminus of the protein you are going to analyze. Ab8637 will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles. I hope this information will help you, please let me know if you need further assistance,

    Read More

    Abcam Scientific Support

    Answered on Sep 09 2005

    Question

    thanks for the information. Is there any information that you have regarding recommended starting concentrations for use of these antibodies as either capture or detection antibodies?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2004

    Answer

    Here is the information that I could obtain regarding ab8639, ab8638, and ab8637. The recommended starting dilutions are 1:1,000 and down in 10-fold increments. As for detection, any known detection method would work. Once again, those mabs had being characterized in great detail in the following paper: Bichko et al. Epitopes recognized by antibodies to denatured core protein of hepatitis B virus. Mol Immunol. 1993 Feb;30(3):221-31 If you have any more questions, just let me know.

    Read More

    Abcam Scientific Support

    Answered on Sep 14 2004

    Question

    thanks for the information. Is there any information that you have regarding recommended starting concentrations for use of these antibodies as either capture or detection antibodies?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2004

    Answer

    I have received the following information regarding ab8255 and ab2045, but will have to get back to you about the other antibodies. The originator has said that they tested the antibodies as pairs in internal ELISA. Possible pairs are H6F5 (ab2045) - H3A4 (ab8255), and H3A4 - H6F5. They have used coating concentration 5 - 10 ug/ml and conjugate concentration 0.2 -1 ug/ml. However, the concentrations need to be optimized separately for every application.

    Read More

    Abcam Scientific Support

    Answered on Sep 13 2004

    Question

    Customer would like more details about how these antibodies were tested for application in ELISA.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 26 2004

    Answer

    Thank you for your enquiry. I was able to obtain the following information regarding the use of these antibodies in ELISA. Ab8255 and Ab2045: These antibodies have been tested in ELISA with the native antigen. The antibodies can be used as a pair in sandwich ELISA. Both clones can work as capture and as detection antibodies. Ab8638, Ab8637, and Ab8639: These antibodies work well with a recombinant or natural HBcAg adsorbed on a plate, or in sandwich ELISA. Also, they work well with the synthetic HBcAg peptides. Below is one reference: Bichko et al. Epitopes recognized by antibodies to denatured core protein of hepatitis B virus. Mol Immunol. 1993 Feb;30(3):221-31

    Read More

    Abcam Scientific Support

    Answered on Sep 07 2004

    Question

    Thanks for your rapid reply. I have one more question. My end user wants to know the type of HBV core antigen.(i.e.ayw1, ayw2...) He is supposed to it's type is ayw. Inform me about that, if you can confirm it. Best Regareds

    Read More

    Abcam community

    Verified customer

    Asked on Sep 07 2004

    Answer

    Thank you for your reply. The only data we have regarding this is that it is an ayw variant as described in: Bichko et. al. Subtype ayw variant of hepatitis B virus. DNA primary structure analysis. FEBS Lett. 1985 Jun 3;185(1):208-12.

    Read More

    Abcam Scientific Support

    Answered on Sep 07 2004

    1-10 of 12 Abreviews or Q&A

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