Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)

Abreviews (1)Q&A (11)Specific References (4)

Overview

  • Product name
    Anti-Hepatitis B Virus Core Antigen antibody [C1]
    See all Hepatitis B Virus Core Antigen primary antibodies
  • Description
    Mouse monoclonal [C1] to Hepatitis B Virus Core Antigen
  • Host species
    Mouse
  • Specificity
    This antibody reacts with HBV Core Antigen (Major antigenic determinant, c1). Ab8637 should recognize both the precoreprotein and core protein. It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles.
  • Tested applications
    Suitable for: ELISA, IHC-Fr, IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Hepatitis B virus
  • Immunogen

    Purified Hepatitis B Core Antigen

  • Epitope
    Around aa positions 70-80, major, or "c1" determinant

Properties

Applications

Our Abpromise guarantee covers the use of ab8637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr 1/100.
IP Use at an assay dependent concentration.
WB 1/1000. We recommend boiling the sample for 10 minutes before loading.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
  • Cellular localization
    Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
  • Database links
    • Alternative names
      • C antibody
      • Capsid protein antibody
      • Core and e antigen antibody
      • core antibody
      • Core antigen antibody
      • Core protein antibody
      • HBc antibody
      • HBcAg antibody
      • HBVgp4 antibody
      • Hepatitis B core antigen antibody
      • Hepatitis B Virus core antigen antibody
      • p21.5 antibody
      • precore/core protein antibody
      see all

    Images

    • Western blot - Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)
      Western blot - Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)

      Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183

      Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183

    References

    This product has been referenced in:
    • Liu B  et al. Hepatitis B virus X protein inhibits autophagic degradation by impairing lysosomal maturation. Autophagy 10:416-30 (2014). Read more (PubMed: 24401568) »
    • Hasmoni SS  et al. Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage. J Gen Appl Microbiol 51:125-31 (2005). Read more (PubMed: 15942873) »

    See all 4 Publications for this product

    Publishing research using ab8637? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    12 reviews or Q&A

    10
    Votes: Highest First

    Please provide C of A ‘s for the following



    ab39716 lot *
    ab8637 lot G*

    ab49013 lot*

    ab58713 lot G*


    Thank you for contacting Abcam.
    Please find attached the requested Certificates of Analysis.
    If there is anything else I can help you with, please let me know.

    The antibody ab8637 in the following order doesn't work well. The detailed informed is included in the attached file. Could you please give us some help?

    Thank you for contacting Abcam regarding ab8637.


    I am sorry that you are experiencing difficulties with this antibody in WB. In order to assist you further, I wanted to clarify several points in your protocol.


    You mention th...

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    Thank you for your reply.
    here is our explain for your questions:
    1. Apparently, a decreased trend was observed in the expression of the
    target protein of interest in the lanes from left to right, and the result
    from the second blot...

    Read More

    Thank you for your reply.

    Upon further investigation, this target contains several phosphorylation sites which may account for the shift in molecular weight. Otherwise, I cannot account for the shift.

    If you would like to try anot...

    Read More

    DESCRIPTION OF THE PROBLEM Non-specific band Wrong band size at the site of 35kD. SAMPLE Cell extract from HepG2.2.15. PRIMARY ANTIBODY A dilution of 1:800 was used for probing with an incubation of overnight at 4℃, then washed in TBST buffer with agit...

    Read More

    Thank you for contacting Abcam regarding ab8637. I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information and image you provided and would like to ask some additional questions. ...

    Read More

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Hepatitis B Virus Core Antigen antibody [C1]

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Huh7 Human Hepatoma cells)
    Specification
    Huh7 Human Hepatoma cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% Saponin
    Read More
    Username

    Abcam user community

    Verified customer

    Submitted Nov 23 2010

    Another question is whether Ab8637 could not distinguish HBeAg and HBcAg in cells or tissues expressing HBV whole genome. I could not totally understand these two sentences "Ab8637 should recognize both the precoreprotein and core protein.It will not r...

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    Thank you for your question. I can confirm that this Hepatitis B Virus Core Antigen antibody, ab8637, will recognize native core particles but not native e antigen. Under denaturing conditions, it willr ecognize all core polypeptides, containing ep...

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    What is the immunogen used to generate ab8638,ab 8639, ab8637, it is meant to recognise the core protein of Hepatitis B, can we confirm this is the core and NOT the precore protein. The customer wants to detect the core protein of Hep B, in particular...

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    Ab8637 and ab8639 should recognize the precorerotein in WB but there is no "cross-reaction" as all proteins are distinguishable by mobility on the gel (i.e they will recognise both the precoreprotein and core protein). AB8638 may or may not recogni...

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    thanks for the information. Is there any information that you have regarding recommended starting concentrations for use of these antibodies as either capture or detection antibodies?

    Here is the information that I could obtain regarding ab8639, ab8638, and ab8637. The recommended starting dilutions are 1:1,000 and down in 10-fold increments. As for detection, any known detection method would work. Once again, those mabs had being c...

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    thanks for the information. Is there any information that you have regarding recommended starting concentrations for use of these antibodies as either capture or detection antibodies?

    I have received the following information regarding ab8255 and ab2045, but will have to get back to you about the other antibodies. The originator has said that they tested the antibodies as pairs in internal ELISA. Possible pairs are H6F5 (ab2045) - H...

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    Customer would like more details about how these antibodies were tested for application in ELISA.

    Thank you for your enquiry. I was able to obtain the following information regarding the use of these antibodies in ELISA. Ab8255 and Ab2045: These antibodies have been tested in ELISA with the native antigen. The antibodies can be used as a pair in...

    Read More

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