Key features and details
- Mouse monoclonal [C1] to Hepatitis B Virus Core Antigen
- Suitable for: ELISA, IHC-Fr, IP, WB, ICC/IF
- Reacts with: Hepatitis B virus
- Isotype: IgG2a
Product nameAnti-Hepatitis B Virus Core Antigen antibody [C1]
See all Hepatitis B Virus Core Antigen primary antibodies
DescriptionMouse monoclonal [C1] to Hepatitis B Virus Core Antigen
This antibody reacts with HBV Core Antigen (Major antigenic determinant, c1). Ab8637 should recognize both the precoreprotein and core protein. It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles.
Ab8637 was raised against serotype ayw but will work with all other genotypes.
Tested applicationsSuitable for: ELISA, IHC-Fr, IP, WB, ICC/IFmore details
Species reactivityReacts with: Hepatitis B virus
Purified Hepatitis B Core Antigen
EpitopeAround aa positions 70-80, major, or "c1" determinant
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferAscitic fluid
Concentration information loading...
PurityTissue culture supernatant
Light chain typekappa
Our Abpromise guarantee covers the use of ab8637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||1/1000. We recommend boiling the sample for 10 minutes before loading.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceHepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
Cellular localizationCapsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
- C antibody
- Capsid protein antibody
- Core and e antigen antibody
Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183
ab8637 has been referenced in 9 publications.
- Li L et al. FoxO4 inhibits HBV core promoter activity through ERK-mediated downregulation of HNF4a. Antiviral Res 170:104568 (2019). PubMed: 31351930
- Sa-Ngiamsuntorn K et al. An Immortalized Hepatocyte-like Cell Line (imHC) Accommodated Complete Viral Lifecycle, Viral Persistence Form, cccDNA and Eventual Spreading of a Clinically-Isolated HBV. Viruses 11:N/A (2019). PubMed: 31623162
- Ganesan M et al. Acetaldehyde suppresses the display of HBV-MHC class I complexes on HBV-expressing hepatocytes. Am J Physiol Gastrointest Liver Physiol 317:G127-G140 (2019). PubMed: 31141391
- Lahlali T et al. Novel Potent Capsid Assembly Modulators Regulate Multiple Steps of the Hepatitis B Virus Life Cycle. Antimicrob Agents Chemother 62:N/A (2018). PubMed: 30012770
- Diab A et al. Polo-like-kinase 1 is a proviral host factor for hepatitis B virus replication. Hepatology 66:1750-1765 (2017). PubMed: 28445592
- Liu B et al. Hepatitis B virus X protein inhibits autophagic degradation by impairing lysosomal maturation. Autophagy 10:416-30 (2014). PubMed: 24401568
- Hasmoni SS et al. Detection and precipitation of hepatitis B core antigen using a fusion bacteriophage. J Gen Appl Microbiol 51:125-31 (2005). PubMed: 15942873
- Preikschat P et al. Expression, assembly competence and antigenic properties of hepatitis B virus core gene deletion variants from infected liver cells. J Gen Virol 80 ( Pt 7):1777-88 (1999). PubMed: 10423147
- Colucci G et al. Identification of a major hepatitis B core antigen (HBcAg) determinant by using synthetic peptides and monoclonal antibodies. J Immunol 141:4376-80 (1988). PubMed: 2461991