Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)
Key features and details
- Mouse monoclonal [C1] to Hepatitis B Virus Core Antigen
- Suitable for: ELISA, IHC-Fr, IP, WB, ICC/IF, IHC-P
- Reacts with: Hepatitis B virus, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-Hepatitis B Virus Core Antigen antibody [C1]
See all Hepatitis B Virus Core Antigen primary antibodies -
Description
Mouse monoclonal [C1] to Hepatitis B Virus Core Antigen -
Host species
Mouse -
Specificity
This antibody reacts with HBV Core Antigen (Major antigenic determinant, c1). Ab8637 should recognize both the precoreprotein and core protein. It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles.
Ab8637 was raised against serotype ayw but will work with all other genotypes.
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Tested applications
Suitable for: ELISA, IHC-Fr, IP, WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Hepatitis B virus, Recombinant fragment -
Immunogen
Tissue, cells or virus corresponding to Hepatitis B virus Hepatitis B Virus Core Antigen. Purified Hepatitis B Core Antigen
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Epitope
Around aa positions 70-80, major, or "c1" determinant -
General notes
This product was raised against native assembled HBV core (HBV capsid, or particulated core).
For denaturing western blots and denaturing Immunofluorescence we would recommend one of the following:
Anti-Hepatitis B Virus Core Antigen antibody [10E11] (ab8639)
Anti-Hepatitis B Virus Core Antigen antibody [14E11] (ab8638)
These products are raised to the same, but denatured, HBcAg protein sequence thus have more robust activity.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
C1 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
1/100.
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IP |
Use at an assay dependent concentration.
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WB |
1/1000.
We recommend boiling (100°C) the sample for 10 minutes immeditaley before loading and using a 2x or 4x Laemmli sample buffer (contains SDS). A 2X concentrated Laemmli buffer is: 4% SDS, 10% beta-mercaeptoethanol, 20% glycerol, 0.1M Tris pH 6.8, and 0.005% of bromophenol blue. |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
We recommend fixation for 20 minutes and incubation with primary antibody for 1 hour. |
Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
1/100. |
IP
Use at an assay dependent concentration. |
WB
1/1000. We recommend boiling (100°C) the sample for 10 minutes immeditaley before loading and using a 2x or 4x Laemmli sample buffer (contains SDS). A 2X concentrated Laemmli buffer is: 4% SDS, 10% beta-mercaeptoethanol, 20% glycerol, 0.1M Tris pH 6.8, and 0.005% of bromophenol blue. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. We recommend fixation for 20 minutes and incubation with primary antibody for 1 hour. |
Target
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Relevance
Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection. -
Cellular localization
Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus. -
Database links
- Entrez Gene: 2828293 Hepatitis B virus
- Entrez Gene: 944568 Hepatitis B virus
- SwissProt: P03146 Hepatitis B virus
- SwissProt: P03147 Hepatitis B virus
- SwissProt: P03148 Hepatitis B virus
- SwissProt: P0C573 Hepatitis B virus
- SwissProt: P69706 Hepatitis B virus
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Alternative names
- C antibody
- Capsid protein antibody
- Core and e antigen antibody
see all
Images
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Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183
Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab8637 has been referenced in 14 publications.
- Lin Y et al. Glucosamine promotes hepatitis B virus replication through its dual effects in suppressing autophagic degradation and inhibiting MTORC1 signaling. Autophagy 16:548-561 (2020). PubMed: 31204557
- Xie M et al. Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF3 ubiquitination through recruiting OTUB1. FEBS J 287:310-324 (2020). PubMed: 31386800
- Tran BM et al. The Hepatitis B Virus Pre-Core Protein p22 Activates Wnt Signaling. Cancers (Basel) 12:N/A (2020). PubMed: 32486480
- Lau KCK et al. Differences in HBV Replication, APOBEC3 Family Expression, and Inflammatory Cytokine Levels Between Wild-Type HBV and Pre-core (G1896A) or Basal Core Promoter (A1762T/G1764A) Mutants. Front Microbiol 11:1653 (2020). PubMed: 32760388
- Morrish E et al. Combinatorial Treatment of Birinapant and Zosuquidar Enhances Effective Control of HBV Replication In Vivo. Viruses 12:N/A (2020). PubMed: 32824616