Overview

  • Product name

    Anti-Hepatitis B Virus Core Antigen antibody [C1]
    See all Hepatitis B Virus Core Antigen primary antibodies
  • Description

    Mouse monoclonal [C1] to Hepatitis B Virus Core Antigen
  • Host species

    Mouse
  • Specificity

    This antibody reacts with HBV Core Antigen (Major antigenic determinant, c1). Ab8637 should recognize both the precoreprotein and core protein. It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles.

    Ab8637 was raised against serotype ayw but will work with all other genotypes. 

  • Tested applications

    Suitable for: ELISA, IHC-Fr, IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Hepatitis B virus
  • Immunogen

    Purified Hepatitis B Core Antigen

  • Epitope

    Around aa positions 70-80, major, or "c1" determinant

Properties

Applications

Our Abpromise guarantee covers the use of ab8637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-Fr 1/100.
IP Use at an assay dependent concentration.
WB 1/1000. We recommend boiling the sample for 10 minutes before loading.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Hepatitis B Virus Core Antigen (HBcAg) is part of the infectious virion containing an inner "core particle" enclosing the viral genome. The icosahedral core particle contains 180 or 240 copies of the core protein. HBcAg is one of the three major clinical antigens of hepatitis B virus but disappears early in the course of infection. The hepatitis B virus core antigen (HBcAg) is a highly immunogenic subviral particle and functions as both a T-cell-dependent and a T-cell-independent antigen. Therefore, HBcAg may be a promising candidate target for therapeutic vaccine control of chronic HBV infection.
  • Cellular localization

    Capsid protein: Virion. Host cytoplasm, hepatocyte nucleus.
  • Database links

    • Alternative names

      • C antibody
      • Capsid protein antibody
      • Core and e antigen antibody
      • core antibody
      • Core antigen antibody
      • Core protein antibody
      • HBc antibody
      • HBcAg antibody
      • HBVgp4 antibody
      • Hepatitis B core antigen antibody
      • Hepatitis B Virus core antigen antibody
      • p21.5 antibody
      • precore/core protein antibody
      see all

    Images

    • Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183

      Immunoblotting analysis of the recombinant HBcAg antigen using ab8637. E.coli lysates were separated using 12% SDS-PAAG electrophoresis, imunoblotted and developed with the Amersham ECL Detection Kit. Samples: 1. Negative control; 2. HBcAg(full length, 1-183

    References

    This product has been referenced in:

    • Li L  et al. FoxO4 inhibits HBV core promoter activity through ERK-mediated downregulation of HNF4a. Antiviral Res 170:104568 (2019). Read more (PubMed: 31351930) »
    • Lahlali T  et al. Novel Potent Capsid Assembly Modulators Regulate Multiple Steps of the Hepatitis B Virus Life Cycle. Antimicrob Agents Chemother 62:N/A (2018). Read more (PubMed: 30012770) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    11-12 of 12 Abreviews or Q&A

    Answer

    Thank you for your reply. The only data we have regarding this is that it is an ayw variant as described in: Bichko et. al. Subtype ayw variant of hepatitis B virus. DNA primary structure analysis. FEBS Lett. 1985 Jun 3;185(1):208-12.

    Read More

    Question
    Answer

    Thank you for your enquiry. Both of the products that you have expressed an interest in recognise HBV core antigen of 21 kDa. If you need any further assistance please do not hesitate to get back in touch.

    Read More

    11-12 of 12 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up