For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
What is the immunogen used to generate ab8638,ab 8639, ab8637, it is meant to recognise the core protein of Hepatitis B, can we confirm this is the core and NOT the precore protein. The customer wants to detect the core protein of Hep B, in particular fragment 1-150 (N term) in WB.
Asked on Sep 07 2005
Ab8637 and ab8639 should recognize the precorerotein in WB but there is no "cross-reaction" as all proteins are distinguishable by mobility on the gel (i.e they will recognise both the precoreprotein and core protein). AB8638 may or may not recognize your protein fragment aa1-150, since its epitope is aa aa35-140 of the core protein, which is really close to the N terminus of the protein you are going to analyze. Ab8637 will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles. I hope this information will help you, please let me know if you need further assistance,
Answered on Sep 09 2005