For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
Another question is whether Ab8637 could not distinguish HBeAg and HBcAg in cells or tissues expressing HBV whole genome. I could not totally understand these two sentences "Ab8637 should recognize both the precoreprotein and core protein.It will not recognize the precorprotein under native conditions, because this protein can not self assemble into particles." in the protocol. Thank you.
Asked on Sep 04 2007
Thank you for your question. I can confirm that this Hepatitis B Virus Core Antigen antibody, ab8637, will recognize native core particles but not native e antigen. Under denaturing conditions, it willr ecognize all core polypeptides, containing epitope around aa 80 (core, precore, e antigen etc.). Differentiation of core and e antigens in liver tissues using this antibody, and several others, has been published in: J Med Virol. 1997 Oct;53(2):127-38. Differentiation of core gene products of the hepatitis B virus in infected liver tissue using monoclonal antibodies.Naoumov NV, Antonov KA, Miska S, Bichko V, Williams R, Will H. This antibody will recognize all core polypeptides (core, precore, e antigen etc.) under standard western blot conditions. It appears that at least partial renaturing of proteins occurs on the membrane, so no additional renaturing steps are necessary. I hope this information is helpful. Should you have any further questions, please do not hesiate to contact us again.
Answered on Sep 04 2007