Question (38874) | Anti-Hepatitis B Virus Core Antigen antibody [C1] (ab8637)

Go to datasheet (ab8637)

Question

Thank you for your reply.
here is our explain for your questions:
1. Apparently, a decreased trend was observed in the expression of the
target protein of interest in the lanes from left to right, and the result
from the second bloting was similar. As a matter of fact, the samples
loaded into the 3th to the 6th lane were treated with an agent under study
with different concentration, and an efficacy as expected was observed but
the resultant band size was significantly larger than the expected (21KD).
2. A SDS-PAGE gel with a percentage of 12% was used for running, and the
samples were boiled for 10 minutes before loading.
we are looking for your reply again

Answer

Thank you for your reply.

Upon further investigation, this target contains several phosphorylation sites which may account for the shift in molecular weight. Otherwise, I cannot account for the shift.

If you would like to try another vial of this antibody, I would be happy to send you one. Please let me know if you are interested.

I look forward to your reply so that I may assist you further. Please do not hesitate to contact us if you have any additional questions.

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