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thanks for the information. Is there any information that you have regarding recommended starting concentrations for use of these antibodies as either capture or detection antibodies?
Asked on Sep 07 2004
I have received the following information regarding ab8255 and ab2045, but will have to get back to you about the other antibodies. The originator has said that they tested the antibodies as pairs in internal ELISA. Possible pairs are H6F5 (ab2045) - H3A4 (ab8255), and H3A4 - H6F5. They have used coating concentration 5 - 10 ug/ml and conjugate concentration 0.2 -1 ug/ml. However, the concentrations need to be optimized separately for every application.
Answered on Sep 13 2004