Overview

  • Product name

    Anti-Hepatitis B Virus pre S2 Antigen antibody [S26]
    See all Hepatitis B Virus pre S2 Antigen primary antibodies
  • Description

    Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen
  • Host species

    Mouse
  • Specificity

    This antibody reacts with HBV Large and Middle Surface Antigen but not small forms (both glycosylated and non glycosylated species).
  • Tested applications

    Suitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Hepatitis B virus
  • Immunogen

    Purified serum HBV surface antigen

  • Epitope

    aa positions 131-139 (adw nomenclature)

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    S26
  • Myeloma

    Sp2/0
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-FoFr
IHC-Fr
Immunomicroscopy
IP
IHC-P
WB
ICC/IF
  • Application notes
    For western blotting use at 1:1000
    For immunohistochemistry use at 1:100
  • Target

    • Relevance

      The human hepatitis B virus (HBV) is a DNA virus that causes chronic infections that frequently lead to the development of cirrhosis and cellular hepatocellular carcinoma. The HBsAg (surface antigen) gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced. The preS2 domain is the minimal functional unit of transcription activators that are encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). Pre-S2 is a diagnostically important surface antigen of human hepatitis B virus. The PreS domain (S1 + S2) of Hepatitis B virus (HBV) surface antigen may be a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes.
    • Cellular localization

      Virion membrane (By similarity).
    • Database links

      • Alternative names

        • HBV pre S2 Antigen antibody
        • HBV pre S2 protein antibody
        • Hepatitis B Virus pre S2 protein antibody
        • PreS2/S antigen antibody
        • S antibody
        see all

      Images

      • All lanes : Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution

        Lane 1 : Whole cell lysate prepared from HEK cells
        Lanes 2-3 : Whole cell lysate prepared from HEK cells transfected with pCI M

        Lysates/proteins at 50 µg per lane.

        Secondary
        All lanes : Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Observed band size: 33,36,39 kDa
        why is the actual band size different from the predicted?


        Exposure time: 10 minutes
      • Immunofluorescence analysis of mutant pHBV-env–transfected Huh-7 cells, staining Hepatitis B Virus pre S2 Antigen with ab8635.

        Cells were fixed with 4% paraformaldehyde and permeabilized with with saponin 0.1%. Cells were incubated with primary antibody (1/500)  for 2 hours at room temperature. An Alexa Fluor®555–conjugated goat anti-mouse IgG (1/1000) was used as the secondary antibody.

      References

      This product has been referenced in:

      • Li YX  et al. Hepatitis B Virus Middle Protein Enhances IL-6 Production via p38 MAPK/NF-?B Pathways in an ER Stress-Dependent Manner. PLoS One 11:e0159089 (2016). Read more (PubMed: 27434097) »
      • Draz MS  et al. Hybrid nanocluster plasmonic resonator for immunological detection of hepatitis B virus. ACS Nano 6:7634-43 (2012). Read more (PubMed: 22934963) »
      See all 4 Publications for this product

      Customer reviews and Q&As

      1-3 of 3 Abreviews or Q&A

      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeK cells)
      Loading amount
      50 µg
      Specification
      HeK cells
      Gel Running Conditions
      Reduced Denaturing (10)
      Blocking step
      Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

      Mrs. Alina Macovei

      Verified customer

      Submitted Apr 15 2011

      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (transfected Huh7)
      Specification
      transfected Huh7
      Blocking step
      BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3

      Miss. Nadia Warner

      Verified customer

      Submitted Feb 07 2006

      Answer

      I can confirm that the antibody ab8635 recognizes large and middle but not small forms of HBsAg. Both glycosylated and non glycosylated species are reactive. Please do not hesitate to contact us again if you need further information,

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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