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  1. Link

    hepatitis-b-virus-pre-s2-antigen-antibody-s26-ab8635.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus Hepatitis B
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Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)

  • Datasheet
Reviews (2)Q&A (1)References (5)

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Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)

    Key features and details

    • Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen
    • Suitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WB
    • Reacts with: Hepatitis B virus
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-Hepatitis B Virus pre S2 Antigen antibody [S26]
    • Description

      Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen
    • Host species

      Mouse
    • Specificity

      This antibody reacts with HBV Large and Middle Surface Antigen but not small forms (both glycosylated and non glycosylated species).
    • Tested applications

      Suitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WBmore details
    • Species reactivity

      Reacts with: Hepatitis B virus
    • Immunogen

      Tissue, cells or virus corresponding to Hepatitis B virus Hepatitis B Virus pre S2 Antigen. Purified serum HBV surface antigen
      Database link: A0FDJ9

    • Epitope

      aa positions 131-139 (adw nomenclature)
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Concentration information loading...
    • Clonality

      Monoclonal
    • Clone number

      S26
    • Myeloma

      Sp2/0
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • DNA Virus
      • double stranded DNA Virus
      • Hepatitis B

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab8635 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    IHC-FoFr
    1/100.
    IHC-Fr
    1/100.
    Immunomicroscopy
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    IHC-P
    1/100.
    WB (2)
    1/1000.
    Notes
    ELISA
    Use at an assay dependent concentration.
    IHC-FoFr
    1/100.
    IHC-Fr
    1/100.
    Immunomicroscopy
    Use at an assay dependent concentration.
    IP
    Use at an assay dependent concentration.
    IHC-P
    1/100.
    WB
    1/1000.

    Target

    • Relevance

      The human hepatitis B virus (HBV) is a DNA virus that causes chronic infections that frequently lead to the development of cirrhosis and cellular hepatocellular carcinoma. The HBsAg (surface antigen) gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced. The preS2 domain is the minimal functional unit of transcription activators that are encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). Pre-S2 is a diagnostically important surface antigen of human hepatitis B virus. The PreS domain (S1 + S2) of Hepatitis B virus (HBV) surface antigen may be a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes.
    • Cellular localization

      Virion membrane (By similarity).
    • Database links

      • Entrez Gene: 2828293 Hepatitis B virus
      • SwissProt: A0FDJ9 Hepatitis B virus
      • Alternative names

        • HBV pre S2 Antigen antibody
        • HBV pre S2 protein antibody
        • Hepatitis B Virus pre S2 protein antibody
        • PreS2/S antigen antibody
        • S antibody
        see all

      Images

      • Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)
        Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)Image courtesy of Alina Macovei by Abreview.
        All lanes : Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution

        Lane 1 : Whole cell lysate prepared from HEK cells
        Lanes 2-3 : Whole cell lysate prepared from HEK cells transfected with pCI M

        Lysates/proteins at 50 µg per lane.

        Secondary
        All lanes : Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Observed band size: 33,36,39 kDa why is the actual band size different from the predicted?


        Exposure time: 10 minutes

      Protocols

      • Immunoprecipitation protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (5)

      Publishing research using ab8635? Please let us know so that we can cite the reference in this datasheet.

      ab8635 has been referenced in 5 publications.

      • Ma A  et al. A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B. Hum Vaccin Immunother 16:756-778 (2020). PubMed: 31687879
      • Li YX  et al. Hepatitis B Virus Middle Protein Enhances IL-6 Production via p38 MAPK/NF-?B Pathways in an ER Stress-Dependent Manner. PLoS One 11:e0159089 (2016). PubMed: 27434097
      • Draz MS  et al. Hybrid nanocluster plasmonic resonator for immunological detection of hepatitis B virus. ACS Nano 6:7634-43 (2012). PubMed: 22934963
      • Villet S  et al. In vitro characterization of viral fitness of therapy-resistant hepatitis B variants. Gastroenterology 136:168-176.e2 (2009). ICC/IF . PubMed: 18996386
      • Glebe D  et al. Pre-s1 antigen-dependent infection of Tupaia hepatocyte cultures with human hepatitis B virus. J Virol 77:9511-21 (2003). PubMed: 12915565

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-Hepatitis B Virus pre S2 Antigen antibody [S26]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeK cells)
      Loading amount
      50 µg
      Specification
      HeK cells
      Gel Running Conditions
      Reduced Denaturing (10)
      Blocking step
      Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
      Read More

      Mrs. Alina Macovei

      Verified customer

      Submitted Apr 15 2011

      Western blot abreview for Anti-Hepatitis B Virus pre S2 Antigen antibody [S26]

      Good
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (transfected Huh7)
      Specification
      transfected Huh7
      Blocking step
      BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3
      Read More

      Miss. Nadia Warner

      Verified customer

      Submitted Feb 07 2006

      Question

      Greetings! Can ab8635 reconize both glycosylated and non-glycosylated form of HBV Large (39/42Kd), Middle (33/36Kd), and Small (24/27Kd) surface antigen? Thank you very much in advance! Best regards,

      Read More

      Abcam community

      Verified customer

      Asked on Feb 21 2005

      Answer

      I can confirm that the antibody ab8635 recognizes large and middle but not small forms of HBsAg. Both glycosylated and non glycosylated species are reactive. Please do not hesitate to contact us again if you need further information,

      Read More

      Abcam Scientific Support

      Answered on Feb 21 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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