Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Hepatitis B Virus pre S2 Antigen antibody [S26]
See all Hepatitis B Virus pre S2 Antigen primary antibodies -
DescriptionMouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen
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Host speciesMouse
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SpecificityThis antibody reacts with HBV Large and Middle Surface Antigen but not small forms (both glycosylated and non glycosylated species).
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Tested applicationsSuitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WB, ICC/IFmore details
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Species reactivityReacts with: Hepatitis B virus
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Immunogen
Purified serum HBV surface antigen
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Epitopeaa positions 131-139 (adw nomenclature)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Concentration information loading... -
ClonalityMonoclonal
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Clone numberS26
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MyelomaSp2/0
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IsotypeIgG1
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab8635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | ||
| IHC-FoFr | ||
| IHC-Fr | ||
| Immunomicroscopy | ||
| IP | ||
| IHC-P | ||
| WB | ||
| ICC/IF |
Application notes
For western blotting use at 1:1000
For immunohistochemistry use at 1:100
For immunohistochemistry use at 1:100
Target
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RelevanceThe human hepatitis B virus (HBV) is a DNA virus that causes chronic infections that frequently lead to the development of cirrhosis and cellular hepatocellular carcinoma. The HBsAg (surface antigen) gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced. The preS2 domain is the minimal functional unit of transcription activators that are encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). Pre-S2 is a diagnostically important surface antigen of human hepatitis B virus. The PreS domain (S1 + S2) of Hepatitis B virus (HBV) surface antigen may be a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes.
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Cellular localizationVirion membrane (By similarity).
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Database links
- Entrez Gene: 2828293 Hepatitis B virus
- SwissProt: A0FDJ9 Hepatitis B virus
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Alternative names
- HBV pre S2 Antigen antibody
- HBV pre S2 protein antibody
- Hepatitis B Virus pre S2 protein antibody
see all
Images
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![Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)](https://www.abcam.com/ps/products/8/ab8635/Images/Hepatitis-B-Virus-pre-S2-Antigen-Primary-antibodies-ab8635-3.jpg)
Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)Image courtesy of Alina Macovei by Abreview.All lanes : Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from HEK cells
Lanes 2-3 : Whole cell lysate prepared from HEK cells transfected with pCI M
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 33,36,39 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes -
![Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)](https://www.abcam.com/ps/products/8/ab8635/Images/ab8635-170919-ab8635refif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)Image from Villet S et al., Gastroenterology, 136, 168-176.e2. Fig 3.; doi: 10.1053/j.gastro.2008.09.068. Epub 2008 Oct 7. with permission from Elsevier.Immunofluorescence analysis of mutant pHBV-env–transfected Huh-7 cells, staining Hepatitis B Virus pre S2 Antigen with ab8635.
Cells were fixed with 4% paraformaldehyde and permeabilized with with saponin 0.1%. Cells were incubated with primary antibody (1/500) for 2 hours at room temperature. An Alexa Fluor®555–conjugated goat anti-mouse IgG (1/1000) was used as the secondary antibody.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Li YX et al. Hepatitis B Virus Middle Protein Enhances IL-6 Production via p38 MAPK/NF-?B Pathways in an ER Stress-Dependent Manner. PLoS One 11:e0159089 (2016). Read more (PubMed: 27434097) »
- Draz MS et al. Hybrid nanocluster plasmonic resonator for immunological detection of hepatitis B virus. ACS Nano 6:7634-43 (2012). Read more (PubMed: 22934963) »
