Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)
![Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)](https://www.abcam.com/ps/products/8/ab8635/Images/ab8635-15077-anti-hepatitis-b-virus-pre-s2-antigen-antibody-s26-western-blot.jpg "Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)")
Key features and details
- Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen
- Suitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WB
- Reacts with: Hepatitis B virus
- Isotype: IgG1
Overview
-
Product name
Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] -
Description
Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen -
Host species
Mouse -
Specificity
This antibody reacts with HBV Large and Middle Surface Antigen but not small forms (both glycosylated and non glycosylated species). -
Tested applications
Suitable for: ELISA, IHC-FoFr, IHC-Fr, Immunomicroscopy, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Purified serum HBV surface antigen
-
Epitope
aa positions 131-139 (adw nomenclature) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
S26 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-FoFr |
1/100.
|
|
| IHC-Fr |
1/100.
|
|
| Immunomicroscopy |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| IHC-P |
1/100.
|
|
| WB | (2) |
1/1000.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-FoFr
1/100. |
|
IHC-Fr
1/100. |
|
Immunomicroscopy
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
IHC-P
1/100. |
|
WB
1/1000. |
Target
-
Relevance
The human hepatitis B virus (HBV) is a DNA virus that causes chronic infections that frequently lead to the development of cirrhosis and cellular hepatocellular carcinoma. The HBsAg (surface antigen) gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced. The preS2 domain is the minimal functional unit of transcription activators that are encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). Pre-S2 is a diagnostically important surface antigen of human hepatitis B virus. The PreS domain (S1 + S2) of Hepatitis B virus (HBV) surface antigen may be a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes. -
Cellular localization
Virion membrane (By similarity). -
Database links
- Entrez Gene: 2828293 Hepatitis B virus
- SwissProt: A0FDJ9 Hepatitis B virus
-
Alternative names
- HBV pre S2 Antigen antibody
- HBV pre S2 protein antibody
- Hepatitis B Virus pre S2 protein antibody
see all
Images
-
Western blot - Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)Image courtesy of Alina Macovei by Abreview.All lanes : Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from HEK cells
Lanes 2-3 : Whole cell lysate prepared from HEK cells transfected with pCI M
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 33,36,39 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
Protocols
Datasheets and documents
-
Datasheet download
References (5)
ab8635 has been referenced in 5 publications.
- Ma A et al. A dendritic cell receptor-targeted chimeric immunotherapeutic protein (C-HBV) for the treatment of chronic hepatitis B. Hum Vaccin Immunother 16:756-778 (2020). PubMed: 31687879
- Li YX et al. Hepatitis B Virus Middle Protein Enhances IL-6 Production via p38 MAPK/NF-?B Pathways in an ER Stress-Dependent Manner. PLoS One 11:e0159089 (2016). PubMed: 27434097
- Draz MS et al. Hybrid nanocluster plasmonic resonator for immunological detection of hepatitis B virus. ACS Nano 6:7634-43 (2012). PubMed: 22934963
- Villet S et al. In vitro characterization of viral fitness of therapy-resistant hepatitis B variants. Gastroenterology 136:168-176.e2 (2009). ICC/IF . PubMed: 18996386
- Glebe D et al. Pre-s1 antigen-dependent infection of Tupaia hepatocyte cultures with human hepatitis B virus. J Virol 77:9511-21 (2003). PubMed: 12915565
