Overview

  • Product name

    Anti-Hepatitis B Virus pre S2 Antigen antibody [S9]
    See all Hepatitis B Virus pre S2 Antigen primary antibodies
  • Description

    Mouse monoclonal [S9] to Hepatitis B Virus pre S2 Antigen
  • Host species

    Mouse
  • Specificity

    Reacts strongly with WHc but not DHBc in ELISA and Western blot. Also reacts with synthetic HBc peptides.
  • Tested applications

    Suitable for: IHC-P, IHC-Fr, WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Hepatitis B virus
  • Immunogen

    Recombinant full length HBcAg (ayw type)

  • Epitope

    amino acids 130-138 of preS2

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    S9
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 31 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    The human hepatitis B virus (HBV) is a DNA virus that causes chronic infections that frequently lead to the development of cirrhosis and cellular hepatocellular carcinoma. The HBsAg (surface antigen) gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced. The preS2 domain is the minimal functional unit of transcription activators that are encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC). Pre-S2 is a diagnostically important surface antigen of human hepatitis B virus. The PreS domain (S1 + S2) of Hepatitis B virus (HBV) surface antigen may be a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes.
  • Cellular localization

    Virion membrane (By similarity).
  • Database links

    • Alternative names

      • HBV pre S2 Antigen antibody
      • HBV pre S2 protein antibody
      • Hepatitis B Virus pre S2 protein antibody
      • PreS2/S antigen antibody
      • S antibody
      see all

    References

    ab65406 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Hepatitis B virus Cell lysate - other (HepG2-NTCP/Primary Human Hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    50 µg
    Treatment
    infection with HBV virion for 7 days
    Specification
    HepG2-NTCP/Primary Human Hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 11 2018

    Answer

    Thank you for contacting us. The epitope recognized by ab65406 has been mapped to amino acids 130-138 of HBV preS2: MCLRRFIIF. I hope this helps, please let me know if you need any additional information.

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