Overview

  • Product name
    Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody [86C]
    See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
  • Description
    Mouse monoclonal [86C] to Hepatitis B Virus Surface Antigen (Ad/Ay)
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Immunogen

    Native HBsAg (ad/ay) protein

  • Positive control
    • HBsAg subtype adw and recombinant HBsAg subtype ayw.

Properties

Applications

Our Abpromise guarantee covers the use of ab20758 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB 1/200. Predicted molecular weight: 25 kDa. See PubMed 19208682. We recommend blocking with milk and incubating ab20758 overnight.

Target

  • Relevance
    Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
  • Cellular localization
    Virion membrane
  • Alternative names
    • HBsAg antibody
    • HBV major surface antigen antibody
    • HBV surface antigen antibody
    • Hepatitis B Virus major surface antigen antibody
    • Major surface antigen antibody
    • S antibody
    see all

References

This product has been referenced in:
  • Fernandez AF  et al. The dynamic DNA methylomes of double-stranded DNA viruses associated with human cancer. Genome Res 19:438-51 (2009). WB ; Human . Read more (PubMed: 19208682) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Tissue lysate - other (analyte spiked into human serum)
Total protein in input
0.003 µg
Immuno-precipitation step
Other - m-270 Epoxy Dynabeads
Specification
analyte spiked into human serum

Patrick Bullard

Verified customer

Submitted Feb 28 2018

Question
Answer

Thank you for contacting us with your enquiry.

We are able to provide certificates of compliance instead of certificates of analysis, so I have prepared the CoC for the lot of ab20758 that we currently have in stock. If you will need a CoC for a different lot, please let me know and I'll be happy to prepare it.

Let me know if you have any questions or if there is anything else that we can do for you. Have a great weekend.

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Answer

I can confirm that the epitope recognized by ab20758 has not been mapped. The immunogen was native HBsAg. The antibody recognizes HBsAg subtypes ad and ay. We unfortunately do not have any information about testing of this antibody with mutant HBsAg. This antibody was tested on recombinant HBsAg subtype adw and recombinant HBsAg subtype ayw. Both antigens represent the full-length HBsAg sequences. These 2 recombinant antigens are not currently in our product list as they have just recently come out of development however if you are interested in purchasing those please let me know and I will do my best to add those to the catalogue for you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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