• Product name

    Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody
    See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
  • Description

    Horse polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay)
  • Host species

  • Specificity

    This antibody is specific for Hepatitis B surface antigen
  • Tested applications

    Suitable for: ELISA, IP, WBmore details
  • Species reactivity

    Reacts with: Hepatitis B virus
  • Immunogen

    Immunization of horses with highly purified Ad/Ay antigen.



Our Abpromise guarantee covers the use of ab9193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 20089655
WB 1/1000. PubMed: 19740987


  • Relevance

    Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
  • Cellular localization

    Virion membrane
  • Database links

    see all
  • Alternative names

    • HBsAg antibody
    • HBV major surface antigen antibody
    • HBV surface antigen antibody
    • Hepatitis B Virus major surface antigen antibody
    • Major surface antigen antibody
    • S antibody
    see all


This product has been referenced in:

  • Huang LH  et al. Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B. Food Technol Biotechnol 57:105-112 (2019). Read more (PubMed: 31316282) »
  • Song J  et al. CRISPR/Cas9-mediated knockout of HBsAg inhibits proliferation and tumorigenicity of HBV-positive hepatocellular carcinoma cells. J Cell Biochem N/A:N/A (2018). Read more (PubMed: 29904948) »
See all 12 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A


The designation Ad and Ay refer to the subtypes of Hepatitis B Surface Antigen. The reference in the product profile simply means that the antigen used was a mixture of both rather than one or the other.

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Unfortunately, I have to be the messenger to confirm that indeed this lot is affected.

Please let me know what kind of compensation your customer would like to receive from us.

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Here are a few specific sELISA protocol details I was able to find:

ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL.

Otherwise, a standard sELISA protocol such as https://www.abcam.com/index.html?pageconfig=resource&rid=11422 (or your current in-house protocol) can be used with appropriate negative controls. One thing I could not find was what source for Hepatitis B Virus Surface Antigen was used in this sELISA.

I hope this is helpful. Please contact me again if you have any further questions.

Use our products? Submit an https://www.abcam.com/abreviews. Earn rewards!

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If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

We have not yet determined which detection antibodies may work with ab9193 in and ELISA.

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Thank you for contacting us.
The antigen was purified from human serum, heat inactivated and injected into horse. The exact immunogen sequence is not known; immunogen contains L, M & S proteins.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Vielen Dank für Ihre Anfrage. Es tut mir leid, dass Sie so lange auf eine Antwort warten mussten. Meine Kollegin Claudia, mit der Sie telefoniert hatten, ist derzeit für Abcam unterwegs und nicht im Büro, hat zuvor jedoch noch genauere Informationen zu ab9193 erfragt. Die Informationen, die uns nun mitgeteilt wurden, besagen, dass die Konzentration von ab9193 photometrisch bestimmt wurde. Die Reinheit des affinitätsaufgereinigten Antikörpers wird leider nicht routinemäßig bestimmt. Wir versuchen nach Möglichkeit alle wichtigen Daten über unsere Produkte an unsere Kunden weiterzugeben und ich möchte mich dafür entschuldigen, dass dies in diesem Fall nicht möglich ist. Ich hoffe, diese Informationen helfen Ihnen dennoch weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Immunocytochemistry/ Immunofluorescence
Human Cell (Huh7 Human Hepatoma Cells)
Huh7 Human Hepatoma Cells
Yes - 0.1% Saponin

Abcam user community

Verified customer

Submitted Mar 22 2011


I would recommend a starting dilution of 1:1500 with this product. Currently we do not have an image available with this antibody but we will provide you with one when it becomes available.

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