Overview

  • Product name

    Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody
    See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
  • Description

    Horse polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay)
  • Host species

    Horse
  • Specificity

    This antibody is specific for Hepatitis B surface antigen
  • Tested applications

    Suitable for: ELISA, IP, WBmore details
  • Species reactivity

    Reacts with: Hepatitis B virus
  • Immunogen

    Immunization of horses with highly purified Ad/Ay antigen.

Properties

Applications

Our Abpromise guarantee covers the use of ab9193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 20089655
WB 1/1000. PubMed: 19740987

Target

  • Relevance

    Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
  • Cellular localization

    Virion membrane
  • Database links

    see all
  • Alternative names

    • HBsAg antibody
    • HBV major surface antigen antibody
    • HBV surface antigen antibody
    • Hepatitis B Virus major surface antigen antibody
    • Major surface antigen antibody
    • S antibody
    see all

References

This product has been referenced in:

  • Huang LH  et al. Development of a Transgenic Flammulina velutipes Oral Vaccine for Hepatitis B. Food Technol Biotechnol 57:105-112 (2019). Read more (PubMed: 31316282) »
  • Song J  et al. CRISPR/Cas9-mediated knockout of HBsAg inhibits proliferation and tumorigenicity of HBV-positive hepatocellular carcinoma cells. J Cell Biochem N/A:N/A (2018). Read more (PubMed: 29904948) »
See all 12 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Huh7 Human Hepatoma Cells)
Specification
Huh7 Human Hepatoma Cells
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Saponin

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Verified customer

Submitted Mar 22 2011

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