Product nameAnti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (Biotin)
See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
DescriptionGoat polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay) (Biotin)
Tested applicationsSuitable for: ICC/IF, ELISAmore details
Species reactivityReacts with: Hepatitis B virus
Mixture of subtypes ad & ay. Hepatitis B surface antigen purified from human serum
Covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
Purification notesPurified IgG fraction of the antiserum covalently coupled with the N-Hydroxysuccinimide ester of Biotin.
Our Abpromise guarantee covers the use of ab20778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: Use at an assay dependent dilution. Use Avidin and streptavidin amplifications systems.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
Cellular localizationVirion membrane
- Entrez Gene: 2828293 Hepatitis B virus
- Entrez Gene: 944569 Hepatitis B virus
- SwissProt: P03138 Hepatitis B virus
- SwissProt: P03140 Hepatitis B virus
- SwissProt: P03141 Hepatitis B virus
- SwissProt: Q81157 Hepatitis B virus
- SwissProt: Q81158 Hepatitis B virus
- SwissProt: Q81159 Hepatitis B virus
- HBsAg antibody
- HBV major surface antigen antibody
- HBV surface antigen antibody
ab20778 has not yet been referenced specifically in any publications.