Key features and details
- Rabbit polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay) (FITC)
- Suitable for: ELISA, ICC/IF
- Reacts with: Hepatitis B virus
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Product nameAnti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (FITC)
See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
DescriptionRabbit polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay) (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
SpecificityThis antibody recognizes Hepatitis B Surface Antigen, subtypes ad and ay.
Tested applicationsSuitable for: ELISA, ICC/IFmore details
Species reactivityReacts with: Hepatitis B virus
Hepatitis B surface antigen purified from human serum. Mixture of subtypes ad and ay.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Purification notesThis antibody was purified to monospecific FITC.
Our Abpromise guarantee covers the use of ab32914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IF: 1/10 - 1/50. Acetone fixation of the antigen source is recommended prior to staining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
Cellular localizationVirion membrane
- Entrez Gene: 2828293 Hepatitis B virus
- Entrez Gene: 944569 Hepatitis B virus
- SwissProt: P03138 Hepatitis B virus
- SwissProt: P03140 Hepatitis B virus
- SwissProt: P03141 Hepatitis B virus
- SwissProt: Q81157 Hepatitis B virus
- SwissProt: Q81158 Hepatitis B virus
- SwissProt: Q81159 Hepatitis B virus
- HBsAg antibody
- HBV major surface antigen antibody
- HBV surface antigen antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab32914 has been referenced in 2 publications.
- Deng Q et al. Hepatitis B virus as a gene delivery vector activating foreign antigenic T cell response that abrogates viral expression in mouse models. Hepatology 50:1380-91 (2009). PubMed: 19821533
- Deng Q et al. Identification and characterization of peptides that interact with hepatitis B virus via the putative receptor binding site. J Virol 81:4244-54 (2007). PubMed: 17192308