Overview

  • Product name

    Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (FITC)
    See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
  • Description

    Rabbit polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay) (FITC)
  • Host species

    Rabbit
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    This antibody recognizes Hepatitis B Surface Antigen, subtypes ad and ay.
  • Tested applications

    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Hepatitis B virus
  • Immunogen

    Hepatitis B surface antigen purified from human serum. Mixture of subtypes ad and ay.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab32914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
ICC/IF
  • Application notes
    ELISA: 1/10000.
    IF: 1/10 - 1/50. Acetone fixation of the antigen source is recommended prior to staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
    • Cellular localization

      Virion membrane
    • Database links

      see all
    • Alternative names

      • HBsAg antibody
      • HBV major surface antigen antibody
      • HBV surface antigen antibody
      • Hepatitis B Virus major surface antigen antibody
      • Major surface antigen antibody
      • S antibody
      see all

    References

    This product has been referenced in:

    • Deng Q  et al. Hepatitis B virus as a gene delivery vector activating foreign antigenic T cell response that abrogates viral expression in mouse models. Hepatology 50:1380-91 (2009). Read more (PubMed: 19821533) »
    • Deng Q  et al. Identification and characterization of peptides that interact with hepatitis B virus via the putative receptor binding site. J Virol 81:4244-54 (2007). Read more (PubMed: 17192308) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Flow Cytometry
    Sample
    Human Cell (HEK293T)
    Permeabilization
    No
    Gating Strategy
    FSC SSC
    Specification
    HEK293T
    Fixation
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 25 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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