Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody [HB24] (ab54247)
Key features and details
- Mouse monoclonal [HB24] to Hepatitis B Virus Surface Antigen (Ad/Ay)
- Suitable for: ELISA
- Reacts with: Hepatitis B virus
- Isotype: IgG1
Overview
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Product name
Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody [HB24]
See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies -
Description
Mouse monoclonal [HB24] to Hepatitis B Virus Surface Antigen (Ad/Ay) -
Host species
Mouse -
Specificity
ab54247 recognises a common epitope of the plasma HBsAg of ad and ay subtypes. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Full length native protein (purified) corresponding to Hepatitis B virus Hepatitis B Virus Surface Antigen (Ad/Ay).
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General notes
Affinity 1.4 x 10-9 M (ad) 2.0 x 10-9 M (ay)The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HB24 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab54247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent dilution.
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Notes |
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ELISA
Use at an assay dependent dilution. |
Target
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Relevance
Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr. -
Cellular localization
Virion membrane -
Database links
- Entrez Gene: 2828293 Hepatitis B virus
- Entrez Gene: 944569 Hepatitis B virus
- SwissProt: P03138 Hepatitis B virus
- SwissProt: P03140 Hepatitis B virus
- SwissProt: P03141 Hepatitis B virus
- SwissProt: Q81157 Hepatitis B virus
- SwissProt: Q81158 Hepatitis B virus
- SwissProt: Q81159 Hepatitis B virus
see all -
Alternative names
- HBsAg antibody
- HBV major surface antigen antibody
- HBV surface antigen antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab54247 has been referenced in 1 publication.
- Draz MS et al. Virus detection using nanoparticles and deep neural network-enabled smartphone system. Sci Adv 6:N/A (2020). PubMed: 33328239