Overview

  • Product name

    Anti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (HRP)
    See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
  • Description

    Rabbit polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay) (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Specificity

    Monospecific, reacts only with Hepatitis B surface antigen including the pre-S1 epitope. Non-reactive with normal human serum.
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Hepatitis B virus
  • Immunogen

    Hepatitis B surface antigen purified from human serum. Mixture of subtypes ad & ay.

  • General notes

    Estimated molar HRP:IgG substitution is 2-3.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.002% Thimerosal (merthiolate)
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Protein A chromatography purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
  • Application notes
    ELISA: Use at an assay dependent dilution.
    Suitability for use in Western blot, IHC and IFA has not been determined but use in such assays should not be excluded.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
    • Cellular localization

      Virion membrane
    • Database links

      see all
    • Alternative names

      • HBsAg antibody
      • HBV major surface antigen antibody
      • HBV surface antigen antibody
      • Hepatitis B Virus major surface antigen antibody
      • Major surface antigen antibody
      • S antibody
      see all

    References

    ab20878 has not yet been referenced specifically in any publications.

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