Product nameAnti-Hepatitis B Virus Surface Antigen (Ad/Ay) antibody (HRP)
See all Hepatitis B Virus Surface Antigen (Ad/Ay) primary antibodies
DescriptionGoat polyclonal to Hepatitis B Virus Surface Antigen (Ad/Ay) (HRP)
SpecificityReacts with purified surface antigen.
Tested applicationsSuitable for: ELISAmore details
Species reactivityReacts with: Hepatitis B virus
Full length native protein (purified) (Hepatitis B virus): Surface Antigen
Free enzyme has been removed. Estimated molar HRP:IgG substitution is 2-3.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.002% Thimerosal (merthiolate)
Constituents: PBS, 10mg/ml BSA. pH 7.2
Concentration information loading...
Purification notes>95% pure.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab34553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/20 - 1/200. Predicted molecular weight: 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
Cellular localizationVirion membrane
- Entrez Gene: 2828293 Hepatitis B virus
- Entrez Gene: 944569 Hepatitis B virus
- SwissProt: P03138 Hepatitis B virus
- SwissProt: P03140 Hepatitis B virus
- SwissProt: P03141 Hepatitis B virus
- SwissProt: Q81157 Hepatitis B virus
- SwissProt: Q81158 Hepatitis B virus
- SwissProt: Q81159 Hepatitis B virus
- HBsAg antibody
- HBV major surface antigen antibody
- HBV surface antigen antibody
ab34553 has not yet been referenced specifically in any publications.