Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted (ab859)

Overview

  • Product name
    Anti-Hepatitis B Virus Surface Antigen antibody [3E7], prediluted
    See all Hepatitis B Virus Surface Antigen primary antibodies
  • Description
    Mouse monoclonal [3E7] to Hepatitis B Virus Surface Antigen, prediluted
  • Host species
    Mouse
  • Specificity
    This antibody stains the cytoplasm of antigen-positive liver cells in patients with Type B viral hepatitis. This antibody reacts with the ‘a’ determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues.
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Hepatitis B virus
  • Immunogen

    BALB/c mice were immunized with purified HBsAG subtype AYW3.

Properties

Applications

Our Abpromise guarantee covers the use of ab859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IHC-Fr
  • Application notes
    This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
  • Target

    • Relevance
      Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
    • Cellular localization
      Virion membrane
    • Database links
      • Alternative names
        • Large envelope protein antibody
        • Large surface protein antibody
        • LHB antibody
        • Major surface antigen antibody

      References

      ab859 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      1-3 of 3 Abreviews or Q&A

      Answer

      Thank you for your email.
      We do offer a variety of antibodies that will recognize the four major serotypes: adr, adw, ayr, ayw.
      If it has been tested which serotype is recognizwed by an antibody this information can be found on the datasheet in the "specificity" section.
      Here is a non- exhaustive list of antibodies that bind all four major serotypes. They have even been characterized further and the datasheet will tell you the serotype subtypes (e.g. ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-)also.
      For example:
      ab2039
      https://www.abcam.com/index.html?datasheet=2039 (or use the following: https://www.abcam.com/index.html?datasheet=2039).
      ab8333
      https://www.abcam.com/index.html?datasheet=8333 (or use the following: https://www.abcam.com/index.html?datasheet=8333).
      ab859
      https://www.abcam.com/index.html?datasheet=859 (or use the following: https://www.abcam.com/index.html?datasheet=859).
      ab20724
      https://www.abcam.com/index.html?datasheet=20724 (or use the following: https://www.abcam.com/index.html?datasheet=20724).
      Which antibody is the most suitable for experiments depends on your samples and the planned application.
      We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about this specific research topic.
      I hope this information is helpful. Please do not hesiate to contact me again with anyfurther questions.

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      Answer

      Regarding ab859 (Hepatitis B Virus Surface Antigen antibody [3E7], prediluted), the concentration of the present lot is 2 ug/ml. Please don't hesitate to contact us again if you have further questions.

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      Answer

      The final concentration of this prediluted antibody is 20ug/ml.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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