• Product name
    Anti-Hepatitis B Virus Surface Antigen antibody [HB12]
    See all Hepatitis B Virus Surface Antigen primary antibodies
  • Description
    Mouse monoclonal [HB12] to Hepatitis B Virus Surface Antigen
  • Host species
  • Specificity
    Reacts with the following HbsAg subtypes : ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-, ayw3 (Fer). 
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Recombinant full length protein (HBsAg of ayw subtype).

  • General notes

    Concentration varies from lot to lot and can be provided on request.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab8636 as a replacement.



Our Abpromise guarantee covers the use of ab2039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.


  • Relevance
    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
  • Cellular localization
    Virion membrane
  • Alternative names
    • Large envelope protein antibody
    • Large surface protein antibody
    • LHB antibody
    • Major surface antigen antibody


ab2039 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A


The exact protein used in the QC ELISA assays is not available. However ab73741 https://www.abcam.com/hepatitis-b-virus-surface-antigen-ayw-protein-ab73741.html would be a good standard for your ELISA.

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Thank you very much for your call this week and for your patience while I have been in touch with the lab regarding yourenquiry.

Each of these antibody pairs was tested at the same concentrations,with the capture antibody coating the plate at5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody at 0.5-5 ug/mL. The assays were done to determine which antibodies could be used together in pairs, and the protocols were not optimized per pair, so we are not sure which will be the most sensitive pair to use.

I am sorry that we do not have more information at this time, but please let me know if you have further questions or if there is anything else that we can do for you.

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Thank you for your email.
We do offer a variety of antibodies that will recognize the four major serotypes: adr, adw, ayr, ayw.
If it has been tested which serotype is recognizwed by an antibody this information can be found on the datasheet in the "specificity" section.
Here is a non- exhaustive list of antibodies that bind all four major serotypes. They have even been characterized further and the datasheet will tell you the serotype subtypes (e.g. ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-)also.
For example:
https://www.abcam.com/index.html?datasheet=2039 (or use the following: https://www.abcam.com/index.html?datasheet=2039).
https://www.abcam.com/index.html?datasheet=8333 (or use the following: https://www.abcam.com/index.html?datasheet=8333).
https://www.abcam.com/index.html?datasheet=859 (or use the following: https://www.abcam.com/index.html?datasheet=859).
https://www.abcam.com/index.html?datasheet=20724 (or use the following: https://www.abcam.com/index.html?datasheet=20724).
Which antibody is the most suitable for experiments depends on your samples and the planned application.
We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about this specific research topic.
I hope this information is helpful. Please do not hesiate to contact me again with anyfurther questions.

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Thank you for your enquiry. I would just like to bring to your attention the fact that ab2039 has a matched pair for ELISA's. If you would like to use ab2039 as the coating antibody, the detection pair for that antibody is ab2042. Ab2042 is an HRP conjugated antibody. Both antibodies are mouse monoclonals, but they recognize different epitopes and have been tested to be a successful match. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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