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  1. Link

    hepatitis-b-virus-surface-antigen-antibody-hb3-ab8256.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus Hepatitis B
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Anti-Hepatitis B Virus Surface Antigen antibody [HB3] (ab8256)

  • Datasheet
  • SDS
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Key features and details

  • Mouse monoclonal [HB3] to Hepatitis B Virus Surface Antigen
  • Suitable for: ELISA, ICC
  • Reacts with: Hepatitis B virus
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Hepatitis B Virus Surface Antigen antibody [HB3]
    See all Hepatitis B Virus Surface Antigen primary antibodies
  • Description

    Mouse monoclonal [HB3] to Hepatitis B Virus Surface Antigen
  • Host species

    Mouse
  • Specificity

    Recognizes following Hepatitis B virus subtypes:ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-. Specificity of MAb HB3 was verified by ELISA on panel of International Workshop on HBsAg Subtypes (Paris, April 1975). This antibody does not cross-block with ab8333 and is therefore suitable for use with ab8333 as a matched pair.
  • Tested applications

    Suitable for: ELISA, ICCmore details
  • Species reactivity

    Reacts with: Hepatitis B virus
  • Immunogen

    Full length native protein (purified from human plasma).

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    HB3
  • Myeloma

    unknown
  • Isotype

    IgG1
  • Light chain type

    unknown
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Hepatitis B
    • Cancer
    • Oncoproteins/suppressors
    • Viral proteins
    • Hepatitis B

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab8256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
ICC
Use at an assay dependent dilution.
Notes
ELISA
Use at an assay dependent concentration.
ICC
Use at an assay dependent dilution.

Target

  • Relevance

    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
  • Cellular localization

    Virion membrane
  • Database links

    • Entrez Gene: 2703546 Hepatitis B virus
    • SwissProt: P03142 Hepatitis B virus
    • Alternative names

      • Large envelope protein antibody
      • Large surface protein antibody
      • LHB antibody
      • Major surface antigen antibody

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab8256? Please let us know so that we can cite the reference in this datasheet.

    ab8256 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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