Anti-Hepatitis B Virus Surface Antigen antibody [HB3] (ab8256)
Key features and details
- Mouse monoclonal [HB3] to Hepatitis B Virus Surface Antigen
- Suitable for: ELISA, ICC
- Reacts with: Hepatitis B virus
- Isotype: IgG1
Overview
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Product name
Anti-Hepatitis B Virus Surface Antigen antibody [HB3]
See all Hepatitis B Virus Surface Antigen primary antibodies -
Description
Mouse monoclonal [HB3] to Hepatitis B Virus Surface Antigen -
Host species
Mouse -
Specificity
Recognizes following Hepatitis B virus subtypes:ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-. Specificity of MAb HB3 was verified by ELISA on panel of International Workshop on HBsAg Subtypes (Paris, April 1975). This antibody does not cross-block with ab8333 and is therefore suitable for use with ab8333 as a matched pair. -
Tested applications
Suitable for: ELISA, ICCmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Full length native protein (purified from human plasma).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
HB3 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab8256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent dilution. |
Target
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Relevance
Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction. -
Cellular localization
Virion membrane -
Database links
- Entrez Gene: 2703546 Hepatitis B virus
- SwissProt: P03142 Hepatitis B virus
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Alternative names
- Large envelope protein antibody
- Large surface protein antibody
- LHB antibody
- Major surface antigen antibody
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References
ab8256 has not yet been referenced specifically in any publications.
