Anti-Hepatitis B Virus Surface Antigen antibody [HB3] (ab8256)
Key features and details
- Mouse monoclonal [HB3] to Hepatitis B Virus Surface Antigen
- Suitable for: ELISA, ICC
- Reacts with: Hepatitis B virus
- Isotype: IgG1
Overview
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Product name
Anti-Hepatitis B Virus Surface Antigen antibody [HB3]
See all Hepatitis B Virus Surface Antigen primary antibodies -
Description
Mouse monoclonal [HB3] to Hepatitis B Virus Surface Antigen -
Host species
Mouse -
Specificity
Recognizes following Hepatitis B virus subtypes:ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-. Specificity of MAb HB3 was verified by ELISA on panel of International Workshop on HBsAg Subtypes (Paris, April 1975). This antibody does not cross-block with ab8333 and is therefore suitable for use with ab8333 as a matched pair. -
Tested applications
Suitable for: ELISA, ICCmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Full length native protein (purified from human plasma).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
HB3 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| ICC |
Use at an assay dependent dilution.
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| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
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ICC
Use at an assay dependent dilution. |
Target
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Relevance
Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction. -
Cellular localization
Virion membrane -
Database links
- Entrez Gene: 2703546 Hepatitis B virus
- SwissProt: P03142 Hepatitis B virus
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Alternative names
- Large envelope protein antibody
- Large surface protein antibody
- LHB antibody
- Major surface antigen antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab8256 has not yet been referenced specifically in any publications.