Anti-Hepatitis B Virus Surface Antigen antibody [HB5] (ab8333)

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Key features and details

  • Mouse monoclonal [HB5] to Hepatitis B Virus Surface Antigen
  • Suitable for: ICC, ELISA
  • Reacts with: Hepatitis B virus
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Hepatitis B Virus Surface Antigen antibody [HB5]
    See all Hepatitis B Virus Surface Antigen primary antibodies

  • Description

    Mouse monoclonal [HB5] to Hepatitis B Virus Surface Antigen

  • Host species

    Mouse

  • Specificity

    Recognizes following Hepatitis B virus subtypes:ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq- Specificity of MAb HB5 was verified by ELISA on panel of International Workshop on HBsAg Subtypes (Paris, April 1975). This antibody does not cross-block with ab8256 and is therefore suitable for use with ab8256 as a matched pair.

  • Tested applications

    Suitable for: ICC, ELISAmore details

  • Species reactivity

    Reacts with: Hepatitis B virus

  • Immunogen

    purified HbsAg from human plasma

Properties

Applications

Our Abpromise guarantee covers the use of ab8333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

  • Cellular localization

    Virion membrane

  • Database links

    • Alternative names

      • Large envelope protein antibody
      • Large surface protein antibody
      • LHB antibody
      • Major surface antigen antibody

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References

    Publishing research using ab8333? Please let us know so that we can cite the reference in this datasheet.

    ab8333 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Hi Abcam Tech Support Team,

    May I know which Human HBsAg Monoclonal antibody (i.e. ab110600, ab54247 and ab20724) binds to all four serotypes of Hepatitis B virus?

    I look forward to receiving your reply soon. Many thanks in advance!

    Best regards,

    Read More
    Answer

    Thank you for your email.
    We do offer a variety of antibodies that will recognize the four major serotypes: adr, adw, ayr, ayw.
    If it has been tested which serotype is recognizwed by an antibody this information can be found on the datasheet in the "specificity" section.
    Here is a non- exhaustive list of antibodies that bind all four major serotypes. They have even been characterized further and the datasheet will tell you the serotype subtypes (e.g. ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-)also.
    For example:
    ab2039
    https://www.abcam.com/index.html?datasheet=2039 (or use the following: https://www.abcam.com/index.html?datasheet=2039).
    ab8333
    https://www.abcam.com/index.html?datasheet=8333 (or use the following: https://www.abcam.com/index.html?datasheet=8333).
    ab859
    https://www.abcam.com/index.html?datasheet=859 (or use the following: https://www.abcam.com/index.html?datasheet=859).
    ab20724
    https://www.abcam.com/index.html?datasheet=20724 (or use the following: https://www.abcam.com/index.html?datasheet=20724).
    Which antibody is the most suitable for experiments depends on your samples and the planned application.
    We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about this specific research topic.
    I hope this information is helpful. Please do not hesiate to contact me again with anyfurther questions.

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    Question

    I am interested in developing an Elisa to quantify mouse antibody titres against HBs virus and I am looking for a source of antibody to use as a positive control, I would be interested if you could tell me more information about the mouse monoclonal antibodies against HBs, and if you supply them in France and their price in euro. Thank you.

    Read More
    Answer

    All the information that we have is available on the datasheets, this includes the price in euros. You can order directly through the Abcam website or if you would prefer you can order from a distributor. There is an ordering section on the Abcam website which has full details, simply click on the flag of your country.

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