Overview

  • Product name
    Anti-Hepatitis B Virus Surface Antigen antibody (HRP)
    See all Hepatitis B Virus Surface Antigen primary antibodies
  • Description
    Goat polyclonal to Hepatitis B Virus Surface Antigen (HRP)
  • Host species
    Goat
  • Conjugation
    HRP
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Non-reactive with normal human serum.
  • Immunogen

    Hepatitis B surface antigen. Mixture of subtypes ad & ay.

Properties

Applications

Our Abpromise guarantee covers the use of ab19990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.

Target

  • Relevance
    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
  • Cellular localization
    Virion membrane
  • Alternative names
    • Large envelope protein antibody
    • Large surface protein antibody
    • LHB antibody
    • Major surface antigen antibody

References

ab19990 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Here are a few specific sELISA protocol details I was able to find:

ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL.

Otherwise, a standard sELISA protocol such as https://www.abcam.com/index.html?pageconfig=resource&rid=11422 (or your current in-house protocol) can be used with appropriate negative controls. One thing I could not find was what source for Hepatitis B Virus Surface Antigen was used in this sELISA.

I hope this is helpful. Please contact me again if you have any further questions.

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Answer



If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

We have not yet determined which detection antibodies may work with ab9193 in and ELISA.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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