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Inquiry: I purchased two anti HBsAg antibodies (ab9193 and ab8636) and ab113610 protein as standard but don't see any signal in my ELISA attempt. Which antibody pairs have you tested for ELISA? Can you recommend a detection Ab that I can use with ab9193, e.g. HRP conjugated ab19990 or ab2042?
Asked on Oct 15 2012
If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.
We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.
We have not yet determined which detection antibodies may work with ab9193 in and ELISA.
Answered on Oct 15 2012