Anti-Hepatitis B Virus Surface Antigen antibody [S14] (ab8636)


  • Product name
    Anti-Hepatitis B Virus Surface Antigen antibody [S14]
    See all Hepatitis B Virus Surface Antigen primary antibodies
  • Description
    Mouse monoclonal [S14] to Hepatitis B Virus Surface Antigen
  • Host species
  • Specificity
    This antibody reacts with HBV Small Surface Antigen
  • Tested applications
    Suitable for: ELISA, IHC-FoFr, IHC-Fr, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Hepatitis B virus
  • Immunogen

    Purified serum HBV surface antigen

  • Epitope



Our Abpromise guarantee covers the use of ab8636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-FoFr 1/100.
IHC-Fr 1/100.
IP Use at an assay dependent concentration.
ICC/IF 1/100.


  • Relevance
    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
  • Cellular localization
    Virion membrane
  • Database links
    • Alternative names
      • Large envelope protein antibody
      • Large surface protein antibody
      • LHB antibody
      • Major surface antigen antibody


    This product has been referenced in:
    • Obeng-Adjei N  et al. DNA vaccine cocktail expressing genotype A and C HBV surface and consensus core antigens generates robust cytotoxic and antibody responses in mice and Rhesus macaques. Cancer Gene Ther 20:652-62 (2013). Read more (PubMed: 24310062) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Here are a few specific sELISA protocol details I was able to find:

    ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL. Read More

    If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

    We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

    We have not yet de...

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    Thank you for your inquiry. I can confirm that this antibody will recognize all three forms of Hepatitis B Virus Surface Antigen: L form (preS1/preS2/S) or M form (preS2/S) or S form (S). So you can expect up to three bands on the SDS gel depen...

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    Thank you for contacting Abcam. I have heard back from the lab about ab8636 and they have told me that the antibody was provided as a bioreactor harvest and so no further purification is needed for any of the assays. They also informed me that protein ...

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    Virtually all anti-HBsAg mabs (including this one) are conformation-specific, i.e., they recognize only native antigen. That's why they do not work usually neither in WB nor in denaturing ELISA. ab18797 will work in both of those assays, but only the...

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