Overview

  • Product name
    Anti-Hepatitis B Virus Surface Antigen antibody [S14]
    See all Hepatitis B Virus Surface Antigen primary antibodies
  • Description
    Mouse monoclonal [S14] to Hepatitis B Virus Surface Antigen
  • Host species
    Mouse
  • Specificity
    This antibody reacts with HBV Small Surface Antigen
  • Tested applications
    Suitable for: ELISA, IHC-FoFr, IHC-Fr, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Hepatitis B virus
  • Immunogen

    Purified serum HBV surface antigen

  • Epitope
    unknown

Properties

Applications

Our Abpromise guarantee covers the use of ab8636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-FoFr 1/100.
IHC-Fr 1/100.
IP Use at an assay dependent concentration.
ICC/IF 1/100.

Target

  • Relevance
    Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.
  • Cellular localization
    Virion membrane
  • Database links
    • Alternative names
      • Large envelope protein antibody
      • Large surface protein antibody
      • LHB antibody
      • Major surface antigen antibody

    References

    This product has been referenced in:
    • Obeng-Adjei N  et al. DNA vaccine cocktail expressing genotype A and C HBV surface and consensus core antigens generates robust cytotoxic and antibody responses in mice and Rhesus macaques. Cancer Gene Ther 20:652-62 (2013). Read more (PubMed: 24310062) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Answer

    Here are a few specific sELISA protocol details I was able to find:

    ab2039, the capture antibody, was coated on the plate at 5 μg/ml in 0.1 M carbonate buffer, and the HRP-conjugated detection antibody, ab2042, was used at 0.5-5 ug/mL.

    Otherwise, a standard sELISA protocol such as https://www.abcam.com/index.html?pageconfig=resource&rid=11422 (or your current in-house protocol) can be used with appropriate negative controls. One thing I could not find was what source for Hepatitis B Virus Surface Antigen was used in this sELISA.

    I hope this is helpful. Please contact me again if you have any further questions.

    Use our products? Submit an https://www.abcam.com/abreviews. Earn rewards!

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    Answer



    If you don't mind providing a more complete protocol for the ELISA assay you ran, I can help you to troubleshoot further.

    We have already tested ab2039 to work successfully with ab2042 in sandwich ELISA.

    We have not yet determined which detection antibodies may work with ab9193 in and ELISA.

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    Answer

    Thank you for your inquiry. I can confirm that this antibody will recognize all three forms of Hepatitis B Virus Surface Antigen: L form (preS1/preS2/S) or M form (preS2/S) or S form (S). So you can expect up to three bands on the SDS gel depending on the expression of the different forms in your samples and the experimental conditions. Unfortunately, we are not further specialized in this particular field and therefore encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests. For general protocol concerning protein analysis I can recommend our comprehensive protocol pages: https://www.abcam.com/index.html?pageconfig=popular_protocols I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

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    Question
    Answer

    Thank you for contacting Abcam. I have heard back from the lab about ab8636 and they have told me that the antibody was provided as a bioreactor harvest and so no further purification is needed for any of the assays. They also informed me that protein concentration was determined by standard methods. If there is anything else I can do to help, please let me know.

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    Answer

    Virtually all anti-HBsAg mabs (including this one) are conformation-specific, i.e., they recognize only native antigen. That's why they do not work usually neither in WB nor in denaturing ELISA. ab18797 will work in both of those assays, but only the HBsAg-L or HBsAg-M (not HBsAg-S) antigens will be recognized. For both S14 and S26 mabs the total HBsAg purified from pooled HBV+ serum was used as immunogen. Both ad and ay subtypes are recognized. ab8636- suitable protein controls should be ab73741 or ab91277.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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