Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)
Key features and details
- Mouse monoclonal [3F6-G10] to Hepatitis B Virus X antigen
- Suitable for: ELISA, IHC-P, IHC-Fr
- Reacts with: Hepatitis B virus
- Isotype: IgG2a
Overview
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Product name
Anti-Hepatitis B Virus X antigen antibody [3F6-G10]
See all Hepatitis B Virus X antigen primary antibodies -
Description
Mouse monoclonal [3F6-G10] to Hepatitis B Virus X antigen -
Host species
Mouse -
Specificity
HB-X antigen (17 kD). We have received mixed reports regarding how ab235 works in Western blot, and we are not able to guarantee this antibody in WB. -
Tested applications
Suitable for: ELISA, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
HB-Xag-Protein A Fusion protein
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified IgG prepared by affinity chromotography on protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3F6-G10 -
Myeloma
unknown -
Isotype
IgG2a -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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|
IHC-P |
1/100. This antibody may not require protein digestion pre-treatment or antigen retrieval on paraffin sections.
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|
IHC-Fr |
1/100.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
1/100. This antibody may not require protein digestion pre-treatment or antigen retrieval on paraffin sections. |
IHC-Fr
1/100. |
Target
-
Relevance
Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC. -
Database links
- SwissProt: O91531 Hepatitis B virus
- SwissProt: Q4R1S1 Hepatitis B virus
- SwissProt: Q91C38 Hepatitis B virus
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Alternative names
- HBV X antigen antibody
- HBx antibody
- HBxAg antibody
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab235 has been referenced in 10 publications.
- Song C et al. Associations Between Hepatitis B Virus Infection and Risk of All Cancer Types. JAMA Netw Open 2:e195718 (2019). PubMed: 31199446
- Hou Z & Quan J Hepatitis B virus X protein increases microRNA-21 expression and accelerates the development of hepatoma via the phosphatase and tensin homolog/phosphoinositide 3-kinase/protein kinase B signaling pathway. Mol Med Rep 15:3285-3291 (2017). PubMed: 28339072
- Zhang Y et al. Hepatitis B virus whole-X and X protein play distinct roles in HBV-related hepatocellular carcinoma progression. J Exp Clin Cancer Res 35:87 (2016). PubMed: 27255760
- Li M et al. Hepatitis B virus X protein promotes renal epithelial-mesenchymal transition in human renal proximal tubule epithelial cells through the activation of NF-?B. Int J Mol Med 38:513-20 (2016). PubMed: 27314843
- Liu L et al. Hepatitis B virus X protein mutant upregulates CENP-A expression in hepatoma cells. Oncol Rep 27:168-73 (2012). PubMed: 21956590
- Yen CJ et al. Hepatitis B Virus X Protein Upregulates mTOR Signaling through IKKß to Increase Cell Proliferation and VEGF Production in Hepatocellular Carcinoma. PLoS One 7:e41931 (2012). PubMed: 22848663
- Kuo CY et al. Apoptosis induced by hepatitis B virus X protein in a CCL13-HBx stable cell line. Oncol Rep 28:127-32 (2012). PubMed: 22552576
- Fernandez AF et al. The dynamic DNA methylomes of double-stranded DNA viruses associated with human cancer. Genome Res 19:438-51 (2009). WB . PubMed: 19208682
- Kuo CY et al. HBx inhibits the growth of CCL13-HBX-stable cells via the GSK-3beta/beta-catenin cascade. Intervirology 51:130-6 (2008). WB, IHC-P ; Human . PubMed: 18552505
- Pál J et al. Immunohistochemical assessment and prognostic value of hepatitis B virus X protein in chronic hepatitis and primary hepatocellular carcinomas using anti-HBxAg monoclonal antibody. Pathol Oncol Res 7:178-84 (2001). WB, IHC-P, ELISA ; Human . PubMed: 11692143