Product nameAnti-Hepatitis B Virus X antigen antibody
See all Hepatitis B Virus X antigen primary antibodies
DescriptionRabbit polyclonal to Hepatitis B Virus X antigen
SpecificityThe amino acid sequence of the recombinant human Hepatitis B Protein X is 100% homologous to the amino acid sequence of the natural Hepatitis B Protein X.
Tested applicationsSuitable for: IHC-P, ELISA, WB, IPmore details
Species reactivityReacts with: Hepatitis B virus
Recombinant full length protein corresponding to Hepatitis B virus Hepatitis B Virus X antigen aa 1-154.
Database link: P12936
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Constituents: 0.58% Sodium chloride, 0.134% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab39716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 19441105|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.|
|IP||Use at an assay dependent concentration.|
RelevanceHepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC.
- HBV X antigen antibody
- HBx antibody
- HBxAg antibody
This product has been referenced in:
- Zhao X et al. HBV infection potentiates resistance to S-phase arrest-inducing chemotherapeutics by inhibiting CHK2 pathway in diffuse large B-cell lymphoma. Cell Death Dis 9:61 (2018). WB . Read more (PubMed: 29352124) »
- Wu Y et al. Hepatitis B virus X protein mediates yes-associated protein 1 upregulation in hepatocellular carcinoma. Oncol Lett 12:1971-1974 (2016). WB ; Human . Read more (PubMed: 27602122) »