Anti-Hepatitis B Virus X antigen antibody (ab39716)
Key features and details
- Rabbit polyclonal to Hepatitis B Virus X antigen
- Suitable for: ELISA, WB, IP
- Reacts with: Hepatitis B virus
- Isotype: IgG
Overview
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Product name
Anti-Hepatitis B Virus X antigen antibody
See all Hepatitis B Virus X antigen primary antibodies -
Description
Rabbit polyclonal to Hepatitis B Virus X antigen -
Host species
Rabbit -
Specificity
The amino acid sequence of the recombinant human Hepatitis B Protein X is 100% homologous to the amino acid sequence of the natural Hepatitis B Protein X. -
Tested applications
Suitable for: ELISA, WB, IPmore details -
Species reactivity
Reacts with: Hepatitis B virus -
Immunogen
Recombinant full length protein corresponding to Hepatitis B virus Hepatitis B Virus X antigen aa 1-154.
Database link: P12936 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituents: 0.58% Sodium chloride, 0.134% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa.
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IP |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 17 kDa. |
IP
Use at an assay dependent concentration. |
Target
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Relevance
Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC. -
Database links
- SwissProt: O91531 Hepatitis B virus
- SwissProt: Q4R1S1 Hepatitis B virus
- SwissProt: Q91C38 Hepatitis B virus
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Alternative names
- HBV X antigen antibody
- HBx antibody
- HBxAg antibody
Protocols
Datasheets and documents
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Datasheet download
References (27)
ab39716 has been referenced in 27 publications.
- Li J et al. lncNBAT1/APOBEC3A is a mediator of HBX-induced chemoresistance in diffuse large B cell lymphoma cells. Mol Ther Nucleic Acids 27:1064-1077 (2022). PubMed: 35228900
- Wei XF et al. Identification of STAU1 as a regulator of HBV replication by TurboID-based proximity labeling. iScience 25:104416 (2022). PubMed: 35663023
- Oda C et al. HBx and YAP expression could promote tumor development and progression in HBV-related hepatocellular carcinoma. Biochem Biophys Rep 32:101352 (2022). PubMed: 36160029
- Niu Y et al. Interaction of Hepatitis B Virus X Protein with the Pregnane X Receptor Enhances the Synergistic Effects of Aflatoxin B1 and Hepatitis B Virus on Promoting Hepatocarcinogenesis. J Clin Transl Hepatol 9:466-476 (2021). PubMed: 34447675
- Ni Z et al. Transcriptomic identification of HBx-associated hub genes in hepatocellular carcinoma. PeerJ 9:e12697 (2021). PubMed: 35036167