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Dear Sir or Madam,
We are a biophysical lab and we are working with recombinantly expressed HBV proteins. To validate some of our recombinant protein we're now looking into co-immunoprecipitation and western blotting. These techniques have never been used in our lab, so I have a few questions. To establish antibody techniques in our lab I am interested in HBx antibodies, but once protocols have been established we'd be interested in antibodies against other HBV proteins as well.
Could you send me a quote for the antibodies listed below?
Anti-6X His tag® antibody [3H2201] (ab15149)
Anti-6X His tag® antibody (ab125262)
Anti-6X His tag® antibody (Agarose) (ab90663)
Anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235)
Anti-Hepatitis B Virus X antigen antibody [X36C] (ab2741)
Anti-Hepatitis B Virus X antigen antibody (ab39716)
Anti-p53 antibody [PAb 240] (ab26)
I'm also interested in secondary antibodies for the use in western blots. Could you recommend a matching secondary anti-mouse antibody for WB use?
WouldRabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) be a good choice?
Reading the posts in your support section I can see that the epitopes of the HBx antibodies are not known. Has anything changed since then?
And my last question would be if you supply any conformation specific antibodies against HBV core protein (HBc).
Asked on Aug 28 2012
Thank you for contacting us and your interest in our products.
The request for a quote for the antibodies mentioned has been passed on to one of my colleagues who will get back to you shortly with this information. In regards to your other questions:
The Rabbit anti-Mouse IgG H&L (HRP) secondary antibody (ab7075) should work well with ab15149, ab2741, ab235 and ab26. You would need a different secondary antibody for all the other antibodies mentioned as they are raised in rabbit. You may wantto consider the Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080). Please bear in mind that we have as yet not tested the anti-Hepatitis B Virus X antigen antibody [3F6-G10] (ab235) in Western blotting or immunoprecipitation. This does not mean that it would not work, simply that we have not tested it ourselves as yet and would therefore not he able to guarantee its performance in these applications.
We have some good resources in setting up both immunoprecipitation and Western blotting experiments from the following link:
Some further troubleshooting tips can also be found here:
If you would like any help with this please do let us know.
I will check if any further information is know about the epitopes recognised by the anti-HBx antibodies mentioned and will get back to you with this information as soon as I have it. I will also look into your final question ifwe have any conformation specific antibodies against HBV core protein (HBc).
As you will be setting up these experiments from scratch, you may be interested in looking at our Western blotting range:
The link above will take you not only to our Optiblot range of gels which provide superior durability and ease of use compared to many standard gels, but also the PVDF and nitrocellulose membranes and Ecl Detection kits we have available.
If you would like any further information on these products please do let us know.
Answered on Aug 28 2012