Overview

  • Product name

    Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin)
    See all Hepatitis C Virus primary antibodies
  • Description

    Mouse monoclonal [NS5B-6] to Hepatitis C Virus (Biotin)
  • Host species

    Mouse
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: WB, ELISA, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Hepatitis C virus
  • Immunogen

    Recombinant fragment of NS5b protein of Hepatitis C Virus genotype 1b, expressed in E. coli

  • Positive control

    • Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA.
  • General notes

    Conjugate: Biotin conjugated [biotin] / [IgG] = 8.7

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Purification notes

    filter-sterilized
  • Clonality

    Monoclonal
  • Clone number

    NS5B-6
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 58 kDa (predicted molecular weight: 70 kDa).
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab18418 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
  • Cellular localization

    Virus surface
  • Database links

    • Alternative names

      • Genome polyprotein antibody
      • HCV antibody
      • Hepatitis C virus polyprotein antibody

    Images

    • Anti-Hepatitis C Virus antibody [NS5B-6] (Biotin) (ab122972) + Lysate of chimp liver cells infected with recombinant
      vaccinia virus containing a HCV genome cDNA

      Predicted band size: 70 kDa
      Observed band size: 58 kDa
      why is the actual band size different from the predicted?



      NS5b protein is detected as a 58kDa band.
    • ab122972, staining Hepatitis C Virus NS5b protein in Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by immunofluorescence.

    References

    This product has been referenced in:

    • Zhang MQ  et al. Differential Effects of Autophagy-Related 10 Protein on HCV Replication and Autophagy Flux Are Mediated by Its Cysteine44 and Cysteine135. Front Immunol 9:2176 (2018). Read more (PubMed: 30319633) »
    • Manabe S  et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). Read more (PubMed: 8291245) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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