Overview

  • Product name

    Anti-Hepatitis C Virus antibody [NS5B-6] (FITC)
    See all Hepatitis C Virus primary antibodies
  • Description

    Mouse monoclonal [NS5B-6] to Hepatitis C Virus (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Specificity

    Specific to Human HCV NS5b protein of genotype 1b. Not tested in other genotypes.
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Hepatitis C virus
  • Immunogen

    A region of NS5b protein of Hepatitis C Virus genotype 1b expressed in E. coli.

  • Positive control

    • Chimp liver cells and cell lysate, infected with recombinant vaccinia virus containing a HCV genome cDNA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    ab122979 is filter-sterilized. Purity >90% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    NS5B-6
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 327 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance

    Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
  • Cellular localization

    Virus surface
  • Database links

    • Alternative names

      • Genome polyprotein antibody
      • HCV antibody
      • Hepatitis C virus polyprotein antibody

    Images

    • Anti-Hepatitis C Virus antibody [NS5B-6] (FITC) (ab122979) + Chimp liver cell lysate, infected with recombinant
      vaccinia virus containing a HCV genome cDNA

      Predicted band size: 327 kDa
      Observed band size: 58 kDa
      why is the actual band size different from the predicted?



      The NS5b protein is detected as a 58-kDa band.
    • ab122979, staining Hepatitis C Virus NS5b protein in Acetone-fixed Chimp liver cells infected with recombinant vaccinia virus containing a HCV genome cDNA by Immunofluorescence.

    References

    This product has been referenced in:

    • Manabe S  et al. Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by NS3. Virology 198:636-44 (1994). Read more (PubMed: 8291245) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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