Overview

  • Product name
    Anti-Hepatitis C Virus Core 1b antibody [C7-50]
  • Description
    Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b
  • Host species
    Mouse
  • Specificity
    Detects hepatitis C virus (HCV) core protein from transfected human and primate cell lines.
  • Tested applications
    Suitable for: IP, Flow Cyt, ICC/IF, IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Hepatitis C virus
  • Immunogen

    Recombinant full length protein (GST-tag) corresponding to Hepatitis C Virus Core 1b.

  • Epitope
    This antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV strains.

Properties

Applications

Our Abpromise guarantee covers the use of ab2740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 20422251
IHC-P Use at an assay dependent concentration. PubMed: 25155355
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines.

Target

  • Relevance
    HCV (Hepatitis C Virus) viral core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. The hepatitis C virus (HCV) core protein represents the first 177 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. The N terminus of the core protein is involved in transcriptional repression. There are over 20 different subtypes of Hepatitis C Virus; HCV type 1b is mostly found in Europe and Asia. The prevalence of HCV type 1b infection has recently decreased, although it still accounts for most HCV-related cirrhosis and hepatocellular carcinoma. High HCV viremia levels and HCV genotype type 1b are independent predictors for poor response to interferon-alpha therapy. HCV core protein is among the most conserved proteins in HCV and is known to induce sensitization of cytotoxic T lymphocytes (CTL). Therefore, it is a prime candidate for a component of a potential HCV vaccine.
  • Cellular localization
    Secreted; Host endoplasmic reticulum membrane; Single-pass membrane protein; Host nucleus; Host cytoplasm.
  • Database links
    • Alternative names
      • Capsid protein C antibody
      • Core protein p19 antibody
      • Core protein p21 antibody
      • Hepatitis C virus core protein antibody

    Images

    • ab2740 staining Hepatitis C Virus Core Antigen in the Huh7-Lunet Cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 37°C. An undiluted Alexa Fluor® 647-conjugated Goat anti-mouse IgG monoclonal was used as the secondary antibody.

      See Abreview

    • Immunofluorescence analysis of Huh-7.5.1 cells infected with either Hepatitis C virus (J6/JFH-C) or Hepatitis C virus defective in RNA polymerase activity (J6/JFH pol-null).
      Hepatitis C Virus Core Antigen (red) was stained with ab2740 at 1/300 dilution, 96 hours after infection. A Cy3®-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody. Nuclei were stained with DAPI (blue).

    References

    This product has been referenced in:
    • von Delft A  et al. The generation of a simian adenoviral vectored HCV vaccine encoding genetically conserved gene segments to target multiple HCV genotypes. Vaccine 36:313-321 (2018). WB . Read more (PubMed: 29203182) »
    • Lin YM  et al. Calcitriol Inhibits HCV Infection via Blockade of Activation of PPAR and Interference with Endoplasmic Reticulum-Associated Degradation. Viruses 10:N/A (2018). Read more (PubMed: 29385741) »
    See all 48 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (human hepatocarcinoma)
    Antigen retrieval step
    None
    Permeabilization
    Yes - H2O2
    Specification
    human hepatocarcinoma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.05% · Temperature: 24°C
    Fixative
    Methanol

    Hwanhee Lee

    Verified customer

    Submitted Jul 01 2016

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Human Cell lysate - whole cell (human liver carcinoma cell line)
    Specification
    human liver carcinoma cell line
    Treatment
    transfection of Core protein expressed vector
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Oct 29 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Huh7-Lunet Cells)
    Specification
    Huh7-Lunet Cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C

    Abcam user community

    Verified customer

    Submitted Feb 19 2013

    Answer

    Thank you for confirming the details of this product.

    I am pleased to provide thetesting protocol for ELISA using this antibody which I have copied below. Please note, this is a guideline only and may require some individual optimization.

    While we do not recommend an ELISA specific dilution for this antibody,a usefulstandard starting point for recommendations for ELISA antibody concentrations is 1-10ug/ml for coating and 1-5ug/ml for detection.

    I hope the following article may also be helpful to you:

    J Med Virol. 1996 Mar;48(3):234-41.
    Characterization of three novel monoclonal antibodies against hepatitis C virus core protein.

    Author(s): Moradpour D, Wakita T, Tokushige K, Carlson RI, Krawczynski K, Wands JR
    (http://www.ncbi.nlm.nih.gov/pubmed/8801283)

    I am sorry to confirm that the cut off point has not been determiened. Calculation of the results and statistics will depend on what you require. I can recommend to review further details in the instructions for the platereader software regarding data analysis. I am sorry we have no more specific information to provide.

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact me.

    General ELISA Protocol

    1. Bind Antigen to Microplate

    a. Dispense 1-5 mg antigen in PBS into each well.
    b. Cover the plate with parafilm and place in the refrigerator overnight.
    c. Remove and discard antigen solution from plate.

    2. Blocking and Primary Antibody Incubation

    a. Block each well in the entire microplate by adding 200 ml of 2.5 mg/ml BSA in PBS to each well.
    b. Allow plate to stand at room temperature for 2 hours.
    c. Remove and discard blocking solution from plate.
    d. Add the appropriate amount of primary antibody to each well and incubate at room temperature for 1 hour.
    e. Remove and discard incubation solution from plate.
    f. Wash each well using TBST (TBS containing 0.1% Tween20). Do NOT cross-contaminate wells.
    g. Repeat washing step for a total of 5 washes.

    3. Secondary Incubation and Detection

    a. Add appropriate amount of secondary antibody solution wells and incubate at room temperature for 1 hour.
    b. Remove and discard secondary antibody solution from plate.
    c. Wash each well using TBST (TBS containing 0.1% Tween20). Do NOT cross-contaminate wells.
    d. Repeat washing step for a total of 5 washes.
    e. Add the appropriate detection reagent, incubate, and stop the color development according to manufacturer’s recommendations.
    f. Read the plate according to manufacturer’s recommendations.

    Read More

    Question
    Answer

    It is not known. From epitope mapping it has been shown to be directed against aa 21-40 of the HCV core region. I would suggest seeing how conserved this sequence is in 1a.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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