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  1. Link

    hepatitis-c-virus-core-1b-antibody-c7-50-ab2740.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus HCV
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Anti-Hepatitis C Virus Core 1b antibody [C7-50] (ab2740)

  • Datasheet
  • SDS
Reviews (3)Q&A (2)References (82)

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Key features and details

  • Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b
  • Suitable for: IP, Flow Cyt, ELISA, WB
  • Reacts with: Hepatitis C virus
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Hepatitis C Virus Core 1b antibody [C7-50]
  • Description

    Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b
  • Host species

    Mouse
  • Specificity

    Detects hepatitis C virus (HCV) core protein from transfected human and primate cell lines.
  • Tested applications

    Suitable for: IP, Flow Cyt, ELISA, WBmore details
  • Species reactivity

    Reacts with: Hepatitis C virus
  • Immunogen

    Recombinant full length protein (GST-tag) corresponding to Hepatitis C Virus Core 1b.

  • Epitope

    This antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV strains.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    C7-50
  • Isotype

    IgG1
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • HCV
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • Hepatitis A/C/E/G

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
Use at an assay dependent concentration.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA
Use at an assay dependent concentration.
WB (1)
Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines.
Notes
IP
Use at an assay dependent concentration.
Flow Cyt
1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines.

Target

  • Relevance

    HCV (Hepatitis C Virus) viral core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. The hepatitis C virus (HCV) core protein represents the first 177 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. The N terminus of the core protein is involved in transcriptional repression. There are over 20 different subtypes of Hepatitis C Virus; HCV type 1b is mostly found in Europe and Asia. The prevalence of HCV type 1b infection has recently decreased, although it still accounts for most HCV-related cirrhosis and hepatocellular carcinoma. High HCV viremia levels and HCV genotype type 1b are independent predictors for poor response to interferon-alpha therapy. HCV core protein is among the most conserved proteins in HCV and is known to induce sensitization of cytotoxic T lymphocytes (CTL). Therefore, it is a prime candidate for a component of a potential HCV vaccine.
  • Cellular localization

    Secreted; Host endoplasmic reticulum membrane; Single-pass membrane protein; Host nucleus; Host cytoplasm.
  • Database links

    • Entrez Gene: 951475 Hepatitis C virus
    • SwissProt: P26662 Hepatitis C virus
    • SwissProt: P26663 Hepatitis C virus
    • SwissProt: Q9WMX2 Hepatitis C virus
    • Alternative names

      • Capsid protein C antibody
      • Core protein p19 antibody
      • Core protein p21 antibody
      • Hepatitis C virus core protein antibody

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (82)

    Publishing research using ab2740? Please let us know so that we can cite the reference in this datasheet.

    ab2740 has been referenced in 82 publications.

    • Zou LL  et al. TGF-ß isoforms inhibit hepatitis C virus propagation in transforming growth factor beta/SMAD protein signalling pathway dependent and independent manners. J Cell Mol Med 25:3498-3510 (2021). PubMed: 33682288
    • Xiao D  et al. Mechanisms of microRNA-142 in mitochondrial autophagy and hippocampal damage in a rat model of epilepsy. Int J Mol Med 47:N/A (2021). PubMed: 33846769
    • Sun HY  et al. Sterol O-acyltransferase 2 chaperoned by apolipoprotein J facilitates hepatic lipid accumulation following viral and nutrient stresses. Commun Biol 4:564 (2021). PubMed: 33980978
    • Ma YY  et al. IL28A protein homotetramer structure is required for autolysosomal degradation of HCV-NS5A in vitro. Cell Death Dis 11:200 (2020). PubMed: 32205851
    • Zhang MQ  et al. A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells. Autophagy 16:2167-2179 (2020). PubMed: 31996071
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    1-3 of 3 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Hepatitis C Virus Core 1b antibody [C7-50]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (human hepatocarcinoma)
    Antigen retrieval step
    None
    Permeabilization
    Yes - H2O2
    Specification
    human hepatocarcinoma
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.05% · Temperature: 24°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Hwanhee Lee

    Verified customer

    Submitted Jul 01 2016

    Western blot abreview for Anti-Hepatitis C Virus Core 1b antibody [C7-50]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (12%)
    Sample
    Human Cell lysate - whole cell (human liver carcinoma cell line)
    Specification
    human liver carcinoma cell line
    Treatment
    transfection of Core protein expressed vector
    Blocking step
    BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 29 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Hepatitis C Virus Core Antigen antibody [C7-50]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Huh7-Lunet Cells)
    Specification
    Huh7-Lunet Cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 19 2013

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