Anti-Hepatitis C Virus Core 1b antibody [C7-50] (ab2740)
- Datasheet
- References (59)
- Protocols
Overview
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Product nameAnti-Hepatitis C Virus Core 1b antibody [C7-50]
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DescriptionMouse monoclonal [C7-50] to Hepatitis C Virus Core 1b
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Host speciesMouse
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SpecificityDetects hepatitis C virus (HCV) core protein from transfected human and primate cell lines.
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Tested applicationsSuitable for: IP, Flow Cyt, ICC/IF, IHC-P, ELISA, WBmore details
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Species reactivityReacts with: Hepatitis C virus
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Immunogen
Recombinant full length protein (GST-tag) corresponding to Hepatitis C Virus Core 1b.
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EpitopeThis antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV strains.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberC7-50
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab2740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| IP | Use at an assay dependent concentration. | |
| Flow Cyt | 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use at an assay dependent concentration. PubMed: 20422251 | |
| IHC-P | Use at an assay dependent concentration. PubMed: 25155355 | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines. |
Target
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RelevanceHCV (Hepatitis C Virus) viral core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. The hepatitis C virus (HCV) core protein represents the first 177 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. The N terminus of the core protein is involved in transcriptional repression. There are over 20 different subtypes of Hepatitis C Virus; HCV type 1b is mostly found in Europe and Asia. The prevalence of HCV type 1b infection has recently decreased, although it still accounts for most HCV-related cirrhosis and hepatocellular carcinoma. High HCV viremia levels and HCV genotype type 1b are independent predictors for poor response to interferon-alpha therapy. HCV core protein is among the most conserved proteins in HCV and is known to induce sensitization of cytotoxic T lymphocytes (CTL). Therefore, it is a prime candidate for a component of a potential HCV vaccine.
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Cellular localizationSecreted; Host endoplasmic reticulum membrane; Single-pass membrane protein; Host nucleus; Host cytoplasm.
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Database links
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P26662 Hepatitis C virus
- SwissProt: P26663 Hepatitis C virus
- SwissProt: Q9WMX2 Hepatitis C virus
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Alternative names
- Capsid protein C antibody
- Core protein p19 antibody
- Core protein p21 antibody
- Hepatitis C virus core protein antibody
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis C Virus Core 1b antibody [C7-50] (ab2740)](https://www.abcam.com/ps/products/2/ab2740/Images/ab2740-6302-anti-hepatitis-c-virus-core-1b-antibody-c7-50-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis C Virus Core 1b antibody [C7-50] (ab2740)This image is courtesy of an anonymous Abreviewab2740 staining Hepatitis C Virus Core Antigen in the Huh7-Lunet Cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1% BSA for 30 minutes at 37°C. Samples were incubated with primary antibody (1/200 in PBS + 1% BSA) for 1 hour at 37°C. An undiluted Alexa Fluor® 647-conjugated Goat anti-mouse IgG monoclonal was used as the secondary antibody.
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![Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis C Virus Core 1b antibody [C7-50] (ab2740)](https://www.abcam.com/ps/products/2/ab2740/Images/ab2740-6303-anti-hepatitis-c-virus-core-1b-antibody-c7-50-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hepatitis C Virus Core 1b antibody [C7-50] (ab2740)Image from Arumugaswami V et al., PLoS Pathog. 2008 Oct;4(10):e1000182. Epub 2008 Oct 17.Fig 7.; doi:10.1371/journal.ppat.1000182; October 17, 2008, PLoS Pathog 4(10): e1000182.Immunofluorescence analysis of Huh-7.5.1 cells infected with either Hepatitis C virus (J6/JFH-C) or Hepatitis C virus defective in RNA polymerase activity (J6/JFH pol-null).
Hepatitis C Virus Core Antigen (red) was stained with ab2740 at 1/300 dilution, 96 hours after infection. A Cy3®-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody. Nuclei were stained with DAPI (blue).
Protocols
References
This product has been referenced in:
- Cao L et al. Identification of serotonin 2A receptor as a novel HCV entry factor by a chemical biology strategy. Protein Cell 10:178-195 (2019). Read more (PubMed: 29542010) »
- Nitta S et al. Impact of novel NS5A resistance-associated substitutions of hepatitis C virus detected in treatment-experienced patients. Sci Rep 9:5722 (2019). Read more (PubMed: 30952914) »
