Anti-Hepatitis C Virus Core 1b antibody [C7-50] (ab2740)
Key features and details
- Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b
- Suitable for: IP, Flow Cyt, ELISA, WB
- Reacts with: Hepatitis C virus
- Isotype: IgG1
Overview
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Product name
Anti-Hepatitis C Virus Core 1b antibody [C7-50] -
Description
Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b -
Host species
Mouse -
Specificity
Detects hepatitis C virus (HCV) core protein from transfected human and primate cell lines. -
Tested applications
Suitable for: IP, Flow Cyt, ELISA, WBmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
Recombinant full length protein (GST-tag) corresponding to Hepatitis C Virus Core 1b.
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Epitope
This antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV strains. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
C7-50 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ELISA |
Use at an assay dependent concentration.
|
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| WB | (1) |
Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines.
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| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
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Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ELISA
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines. |
Target
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Relevance
HCV (Hepatitis C Virus) viral core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. The hepatitis C virus (HCV) core protein represents the first 177 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. The N terminus of the core protein is involved in transcriptional repression. There are over 20 different subtypes of Hepatitis C Virus; HCV type 1b is mostly found in Europe and Asia. The prevalence of HCV type 1b infection has recently decreased, although it still accounts for most HCV-related cirrhosis and hepatocellular carcinoma. High HCV viremia levels and HCV genotype type 1b are independent predictors for poor response to interferon-alpha therapy. HCV core protein is among the most conserved proteins in HCV and is known to induce sensitization of cytotoxic T lymphocytes (CTL). Therefore, it is a prime candidate for a component of a potential HCV vaccine. -
Cellular localization
Secreted; Host endoplasmic reticulum membrane; Single-pass membrane protein; Host nucleus; Host cytoplasm. -
Database links
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P26662 Hepatitis C virus
- SwissProt: P26663 Hepatitis C virus
- SwissProt: Q9WMX2 Hepatitis C virus
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Alternative names
- Capsid protein C antibody
- Core protein p19 antibody
- Core protein p21 antibody
- Hepatitis C virus core protein antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (82)
ab2740 has been referenced in 82 publications.
- Zou LL et al. TGF-ß isoforms inhibit hepatitis C virus propagation in transforming growth factor beta/SMAD protein signalling pathway dependent and independent manners. J Cell Mol Med 25:3498-3510 (2021). PubMed: 33682288
- Xiao D et al. Mechanisms of microRNA-142 in mitochondrial autophagy and hippocampal damage in a rat model of epilepsy. Int J Mol Med 47:N/A (2021). PubMed: 33846769
- Sun HY et al. Sterol O-acyltransferase 2 chaperoned by apolipoprotein J facilitates hepatic lipid accumulation following viral and nutrient stresses. Commun Biol 4:564 (2021). PubMed: 33980978
- Ma YY et al. IL28A protein homotetramer structure is required for autolysosomal degradation of HCV-NS5A in vitro. Cell Death Dis 11:200 (2020). PubMed: 32205851
- Zhang MQ et al. A new transcription factor ATG10S activates IFNL2 transcription by binding at an IRF1 site in HepG2 cells. Autophagy 16:2167-2179 (2020). PubMed: 31996071